Laboratory DNA science : an introduction to recombinant DNA techniques and methods of genome analysis
著者
書誌事項
Laboratory DNA science : an introduction to recombinant DNA techniques and methods of genome analysis
Benjamin/Cummings Pub. Co., c1996
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注記
Includes bibliographical references
内容説明・目次
内容説明
This one-of-a-kind manual offers twenty-three foolproof labs designed to make molecular biology accessible and interesting to beginning biology students. Covering the basic techniques of gene manipulation and analysis, these "tried and true" experiments were tested and re-tested by the experienced author team to ensure absolute accuracy and ease of use.
目次
I: BASIC TECHNIQUES.
Labs.
1. Measurements, Micropipetting, and Sterile Techniques. 2. Basic Culture Techniques. 3. DNA Restriction and Electrophoresis.II: DNA MODIFICATION.
4. Effects of DNA Methylation on Restriction.
III: PLASMID TRANSFORMATION AND IDENTIFICATION.
5. Rapid Colony Transformation of E. coli with Plasmid DNA.
6. Purification and Identification of Plasmid DNA.
IV: PLASMID RECOMBINATION AND IDENTIFICATION.
7. Recombination of Antibiotic Resistance Genes.
8. Transformation of E. coli with Recombinant DNA.
9. Replicating to Identify Mixed E. coli Populations.
10. Purification and Identification of Recombinant Plasmid DNA.
V: RESTRICTION MAPPING OF LINEAR AND CIRCULAR CHROMOSOMES.
11. Restriction Mapping of the l Chromosome.
12. Restriction Mapping of the Plasmid pBR322.
VI: SOUTHERN HYBRIDIZATION.
13. Southern Hybridization of l DNA.
VII: GENOMIC LIBRARY CONSTRUCTION AND ANALYSIS.
14. Construction of Genomic Library of l DNA.
15. Transformation of E. coli with l Library.
16. Colony Hybridization of l Library.
17. Purification and Identification of l Clones.
VIII: CLONING BY POLYMERASE CHAIN REACTION.
18. Amplification and Purification of l DNA Fragment.
19. Recombination of PCR Product and Plasmid pBLU.
20. Transformation of E. coli with PCR/pBLU Product.
21. The Purification and Identification of PCR/pBLU Recombinants.
IX: HUMAN DNA FINGERPRINTING.
22. Detection of an Alu Insertion Polymorphism by Polymerase Chain Reaction.
23. Detection of a VNTR Polymorphism by Polymerase Chain Reaction.
Appendix I: Equipment, Supplies, and Reagents.
Appendix II: Recipes for Media, Reagents, and Stock Solutions.
Appendix III: Restriction Map Data for pAMP, pKAN, pBLU, l and Bacteriophage.
Appendix IV: Sequence Data for pAMP, pKAN, pBLU, and Bacteriophage l.
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