Bioconjugation : protein coupling techniques for the biomedical sciences
著者
書誌事項
Bioconjugation : protein coupling techniques for the biomedical sciences
Grove's Dictionaries Inc., 1998 , Macmillan, 1998
- Grove's Dictionaries
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注記
Includes bibliographies and index
内容説明・目次
内容説明
Recent years have seen an enormous increase in the use of protein reagents in the biological and medical sciences. Enzyme conjugates and immobilised proteins are fundamental to many diagnostic applications, and existing therapeutic advances are being made using a range of approaches based on antibody conjugates. Protein conjugation techniques are central to the development of these reagents. This book offers invaluable guidance for the design of protein conjugates of all types. It covers the coupling of proteins to organic molecules, nucleic acids and solid phases as well as other proteins. Written by two experts in the development and optimisation of protein conjugation techniques, it details experimental information otherwise scattered throughout the literature. Practically orientated towards protocol development, it examines the subject from basic protein labelling chemistry to the most recent advances in recombinent technology. Introductory chapters outline the theoretical aspects of proteins and protein coupling, whilst concluding chapters address commercial aspects such as protein preservation, stability and manufacturability of processes.
目次
Introduction - Applications - Protein Chemistry - Enzymes - Antibodies - Protein-Protein Coupling - Protein-Small Molecule Coupling - Protein-DNA Coupling - Protein-Solid Phase & others - Molecular Biology Approaches - Purification and Characterisation - Stability of Protein Reagents - Manufacturability of Processes - Index
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