Gel electrophoresis of proteins : a practical approach

Bibliographic Information

Gel electrophoresis of proteins : a practical approach

edited by B.D. Hames

(The practical approach series, 197)

Oxford University Press, 1998

3rd ed

  • hbk.
  • pbk.

Available at  / 18 libraries

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Note

Includes bibliographical references (p. [319]-343) and index

Description and Table of Contents

Description

This text presents the best methods, hints and tips for core procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with advice to ensure that each method works first time in the reader's hands. of The book is intended for researchers and postgraduates in biochemistry, molecular biology, cell biology, medicine, and immunology.

Table of Contents

  • One-dimensional page
  • protein detection
  • preparative gel electrophoresis
  • capillary SDS electrophoresis
  • conventional isoelectric focusing
  • 2-D gel electrophoresis
  • peptide mapping
  • sequence analysis of gel-resolved proteins
  • analysis of glycoproteins
  • protein - protein interactions. Appendices: list of suppliers
  • bibliography of polypeptide detection methods.

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