Electron microscopy methods and protocols
著者
書誌事項
Electron microscopy methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 117）
Humana Press, c1999
- acid-free paper
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryofixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques - each optimized for reproducibility and robust results - today's gold-standard laboratory manual.
目次
General Preparation of Material and Staining of Sections, Heather A. Davies. Negative Staining of Thinly Spread Biological Particulates, J. Robin Harris. Preparation of Thin-Film Frozen-Hydrated/Vitrified Biological Specimens for Cryoelectron Microscopy, J. Robin Harris and Marc Adrian. The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Imunocytochemistry, Paul Webster. High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling, Kent McDonald. The Application of LR Gold Resin for Immunogold Labelling, J. R. Thorpe. Low-Temperature Embedding in Acrylic Resins, Pierre Gounon. Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections, Catherine Rabouille. Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol, Rick T. Giberson and Richard S. Demaree, Jr. Electron Microscopic Enzyme Cytochemistry, Nobukazu Araki and Tanenori Hatae. In Situ Molecular Hybridization Techniques for Ultrathin Sections, Jean-Guy Forunier and Francoise Escaig-Haye. Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study, M. A. Nasser Hajibagheri, Kenneth Sawin, Steve Gschmeissner, Ken Blight, and Carol Upton. Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy, M. A. Nasser Hajibagheri. Application of Electron Microscopy for Studying Protein-DNA Complexes, Maria Schnos and Ross B. Inman. X-Ray Miocroanalysis Techniques, A. John Morgan, Carole Winters, and Stephen Sturzenbaum. Index.
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