Tissue engineering methods and protocols
著者
書誌事項
Tissue engineering methods and protocols
（Methods in molecular medicine / John M. Walker, series editor, 18）
Humana Press, c1999
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered.
目次
Part I. Materials. Preparation of Collagen-Glycosaminoglycan Copolymers for Tissue Regeneration Lila Chamberlain and Ioannis V. Yannas. Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology Susan J. Sofia Philip R Kuhl and Linda G. Griffith. Nondestructive Evaluation of Biodegradable Porous Matrices for Tissue Engineering Leoncio Garrido. Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering Peter X. Ma and Robert Langer. Formation of Highly Porous Polymeric Foams with Controlled Release Capability: A Phase-Separation Technique S. Kadiyala H. Lo and K. Leong. Magnetic-Induced Alignment of Collagen Fibrils in Tissue Equivalents Timothy S. Girton Nerendra Dubey and Robert T. Tranquillo. Preparation and Use of Thermosensitive Polymers Yi-Lin Cao Clemente Ibarra and Charles Vacanti. In Situ Photopolymerized Hydrogels for Vascular and Peritoneal Wound Healing Amarpreet S. Sawhney and Jeffrey A. Hubbell. Fabrication and Implantation of Gel-Filled Nerve Guidance Channels Ravi V. Bellamkonda and Robert F. Valentini. Small Animal Surgical and Histological Procedeures for Characterizing the Performance of Tissue-Engineered Bone Grafts Kenneth S. James Mark C. Zimmerman and Joachim Kohn. Preparation and Use of Porous Poly(a- Hydroxyester) Scaffolds for Bone Tissue Engineering Anna C. Jen Susan J. Peter and Antionios G. Mikos. Part II. Cells. Quantitative Assessment of Autocrine Cell Loops Gregory Oehrtman Laura Walker Birgit Will Lee Opresko H. StevenWiley and Douglas A. Lauffenburger. Measurement of Recovery of Function Following Whole Muscle Transfer Myoblast Transfer and Gene Therapy John A. Faulkner Susan V. Brooks and Robert G. Dennis. Preparation of Immortilized Human Chondrocyte Cell Lines James R. Robbins and Mary B. Goldring. Part III. Clinical Applications. Isolation and In Vitro Proliferation of Chondrocytes Tenocytes and Ligament Cells Sonya Shortkroff and Myron Spector. Culture and Identification of Autologous Human Articular Chondrocytes for Implantation Ross Tubo and Francois Binette. Organogenesis of Skeletal Muscle in Tissue Culture Herman Vandenburgh Janet Shansky Michael Del Tatto and Joseph Chromiak. Hepatocyte Primary Cultures: Currently Used Systems and Their Applications for Studies of Hepatocyte Growth and Differentiation George K. Michalopoulos. Formation and Characterization of Hepatocyte Spheroids Julie R. Friend Florence J. Wu Linda K. Hansen Rory P. Remmel and Wei-Shou Hu. Isolation and Culture of Human Endothelial Cells Fran+ois Berthiaume. Isolation and Culture of Microvascular Endothelial Cells Lisa Richard Paula Velasco and Michael Detmar. Initiation Maintenance and Quantification of Human Hematopoietic Cell Cultures Paul C. Collins Sanjay D. Patel William M. Miller and E. Terry Papoutsakis. Methods to Isolate Culture and Study Osteoblasts Mechteld V. Hillsley. Cryopreservation of Rat Hepatocytes in a Three-Dimensional Culture Configuration Using a Controlled-Rate Freezing Device Michael J. Russo and Mehmet Toner. Part IV. Cell Material Composites. Microencapsulation of Enzymes Cells and Genetically Engineered Microorganisms Thomas M. S. Chang. Methods for Microencapsulation with HEMA-MMA Shahab Lahooti and Michael V. Sefton. Micropatterning Cells in Tissue Engineering Sangeeta N. Bhatia Martin L. Yarmush and Mehmet Toner. Methods for the Serum-Free Culture of Keratinocytes and Transplantation of Collagen-GAG-Based Skin Substitutes Steven T. Boyce. Use of Skin Equivalent Technology in a Wound Healing Model Michael A. Vaccariello Ashkan Javaherian Nancy Parenteau and Jonathan A. Garlick. Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis Daniel A. Medalie and Jeffrey R. Morgan. Development of a Bioartificial Liver Device Linda K. Hansen Julie R. Friend Rory Remmel Frank B. Cerra and Wei-Shou Hu. Methods for the Implantation of Liver Cells Stephen S. Kim Hirofumi Utsunomiya and Joseph P. Vacanti. Isolation and Long-Term Maintenance of Adult Rat Hepatocytes in Culture Fran+ois Berthiaume Ronald G. Tompkins and Martin L. Yarmush. Design and Fabrication of a Small Caliper Hybrid Arterial Bioprosthesis Gilbert J. L'Italien and William M. Abbott. Methods for the Immunoisolation and Transplantation of Pancreatic Cells Anthony M. Sun. Methods for the Study of Nerve Cell Migration and Patterning Helen M. Buettner and Hsin-Chien Tai. Part V. Measurement Techniques. Estimating Number and Volume of Islets Transplanted within a Planar Immunobarrier Diffusion Chamber Kazuhisa Suzuki Clark K. Colton Susan Bonner-Weir Jennifer Hollister and Gordon C. Weir. Quantitative Measurement of Cell-Cell Adhesion Under Flow Conditions Carroll L. Ramos and Michael B. Lawrence. Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation R. Gregory Allen Solomon R. Eisenberg and Martha L. Gray. Measuring Receptor-Mediated Cell Adhesion Under Flow: Cell-Free Systems Daniel A. Hammer and Debra K. Brunk. In Vitro and In Vivo Quantification of Adhesion Between Leukocytes and Vascular Endothelium Rakesh K. Jain Lance L. Munn Dai Fukumura and Robert J. Melder. Quantitative Measurement of Shear-Stress Effects on Endothelial Cells Maria Papadaki and Larry V. McIntire. Quantitative Modeling of Limitations Caused by Diffusion Athanassios Sambanis and Sanda A. Tan. Index.
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