Molecular bacteriology : protocols and clinical applications

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書誌事項

Molecular bacteriology : protocols and clinical applications

edited by Neil Woodford and Alan P. Johnson

（Methods in molecular medicine / John M. Walker, series editor, 15）

Humana Press, c1998

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

目次

The Impact of Molecular Methods on Clinical Bacteriology R. C. George Genomic DNA Digestion and Ribotyping J. Z. Jordens Pulsed-Field Gel Electrophoresis M. E. Kaufmann Plasmid Analysis A. P. Johnson and N. Woodford DNA Amplification J. P. Clewley and N. Saunders Arbitrarily Primed PCR Methods for Studying Bacterial Diseases D. Ralph and M. McClelland Genomic Fingerprinting by Application of rep-PCR A. M. Ridley Molecular Approaches to the Identification of Streptococci R. E. McLaughlin and J. J. Ferretti Pneumococcal Diseases A. M. Smith and K. P. Klugman Tuberculosis & Other Diseases Caused by Mycobacterium spp M. Goyal and D. B. Young Diptheria A. Efstratiou, K. E. Engler, and A. de Zoysa Infection Caused by Legionella spp T. G Harrison and N.K. Fry Haemophilus Influenzae M. A. Herbert, D. Crook, and E. R. Moxon Meningococcal Infection M. C. J. Maiden Gonorrhoea C. A. Ison Chancroid S. A. Morse, D. L. Trees, and P. A. Totten Mycoplasma/Ureaplasma Infection C. B. Gilroy and D. T. Robinson Salmonella Infections E. John Threlfall, M. D. Hampton and A.M. Ridley Cholera T. J. Barrett and D. N. Cameron Diagnosis and Investigation of Diarrheagenic Escherichia coli J. P. Nataro and J. Martinez Campylobacter Infection J. Gibson and R. J. Owen Helicobacter pylori R. J. Owen and J. Gibson Staphylococci A. Deplano and M. Struelens Application of Molecular Techniques to the Study of Nosocomial Infection Caused by Enterococci T. M. Coque, P. Seetulsingh, K. V. Singh, and B. E. Murray Molecular Approaches for the Detection and Identification of ss-Lactamases D. J. Payne and C. J.Thomson Biochemical and Enzyme Kinetic Applications for the Characterization of ss-Lactamases D. J. Payne and T. H. Farmer ss-lactam Resistance Mediated by Changes in Penicillin-Binding Proteins C. G. Dowson and T. J. Coffey Application of Molecular Techniques for the Study of Aminoglycoside Resistance K. J. Shaw, F. J. Sabatelli, L. Naples, P. Mann, R. S. Hare, and G. H. Miller Molecular Investigation of Glycopeptide Resistance in Gram-Positive Bacteria N. Woodford and J. M. Stigter Quinolone Resistance J. Brown and S. G. B. Amyes Resistance to Tetracyclines, Macrolides, Trimethoprim, and Sulfonamides M. C. Roberts Index

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