CiNii 図書 - Transcription factors : a practical approach

Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library. Chapter 6 deals with identification of transcription factors based on sequence homology analysis by both experimental screening and database searches. Chapter 7 is a new chapter that describes methods of identifying the target genes of a previously uncharacterized factor. The next chapters deal with analysis of transcription factor function. Chapter 8 deals with general techniques, and then the following chapters cover the specialized techniques of in vitro transcription assays using transcriptionally active nuclear extracts derived from rat brain, and analysis of the effect of transcription factors on chromatin structure. The final chapter describes methods for detecting the phosphorylation and glycosylation state of transcription factors.

1. The DNA mobility shift assay
2. Footprint analysis of DNA-protein complexes in vitro and in vivo
3. In vitro transcription and characterization of transcription factors
4. Purification and cloning of DNA binding transcription factors
5. Cloning transcription factors from a cDNA expression library
6. Cloning transcription factors by sequence similarity
7. Identification of target genes for a transcription factor by genomic binding site cloning
8. Analysis of cloned factors
9. Neuronal promoter analysis by in vitro transcription using nuclear extracts from rat brain
10. Preparation of chromatin templates for transcription studies
11. Analysis of transcription factor modifications
Appendix: Suppliers of specialist items

巻冊次: : hbk. ISBN 9780199636976

内容説明

Since the publication of the first edition, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This edition has been updated to provide a comprehensive guide to the methods available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library.

1: The DNA mobility shift assay
2: Footprint analysis of DNA-protein complexes in vitro and in vivo
3: In vitro transcription and characterization of transcription factors
4: Purification and cloning of DNA binding transcription factors
5: Cloning transcription factors from a cDNA expression library
6: Cloning transcription factors by sequence similarity
7: Identification of target genes for a transcription factor by genomic binding site cloning
8: Analysis of cloned factors
9: Neuronal promoter analysis by in vitro transcription using nuclear extracts from rat brain
10: Preparation of chromatin templates for transcription studies
11: Analysis of transcription factor modifications
Appendix: Suppliers of specialist items.

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Transcription factors : a practical approach

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