Short protocols in molecular biology : a compendium of methods from Current protocols in molecular biology

著者

書誌事項

Short protocols in molecular biology : a compendium of methods from Current protocols in molecular biology

editorial board, Frederick M. Ausubel ... [et al.]

Wiley, c1999

4th ed

タイトル別名

Current protocols in molecular biology

大学図書館所蔵 件 / 全40件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Short Protocols in Molecular Biology, Fourth EditionThe Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include:Informatics for Molecular BiologistsAnalysis of Protein InteractionsEpitope TaggingMathematics and Statistics for Molecular BiologistsShort Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods.

目次

Escherichia Coli, Plasmids, and Bacteriophages. Preparation and Analysis of DNA. Enzymatic Manipulation of DNA and RNA. Preparation and Analysis of RNA. Construction of Recombinant DNA Libraries. Screening of Recombinant DNA Libraries. DNA Sequencing. Mutagenesis of Cloned DNA. Introduction of DNA into Mammalian Cells. Analysis of Proteins. Immunology. DNA-Protein Interactions. Saccharomyces Cerevisiae. In Situ Hybridization and Immunohistochemistry. The Polymerase Chain Reaction. Protein Expression. Analysis of Protein Phosphorylation. Sequence Similarity Searching Using the Blast Family of Programs. Analysis of Protein Interactions. Appendices. Index.

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