Tetrahymena thermophila
著者
書誌事項
Tetrahymena thermophila
（Methods in cell biology / edited by David M. Prescott, v. 62）
Academic Press, c2000
- : hb.
- : pbk.
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注記
Includes bibliographical references
内容説明・目次
- 巻冊次
-
: pbk. ISBN 9780120645251
内容説明
Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena as the experimental system of choice.
It contains both theoretical and practical issues in 30 chapters contributed by the world authorities on Tetrahymena; overviews the history, cell biology, and genetics of the organism; describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope; illustrates how the methods can be applied to solve various cell biological problems; and, reviews recently developed strategies for altering gene expression.
目次
D.L. Nanney and E.M. Simon, Laboratory and Evolutionary History of Tetrahymena Thermophila. J. Frankel, Cell Biology of Tetrahymena Thermophila. K.M. Karrer, Tetrahymena Genetics: Two Nuclei are Better Than One. E. Orias, E.P. Hamilton, and J.D. Orias, Tetrahymena as a Laboratory Organism: Useful Strains, Cell Culture, and Cell Line Maintenance. P. Bruns, H.R. Smith, and D. Cassidy-Hanley, Long-term Storage. E.P. Hamilton and E. Orias, Genetic Crosses: Setting up Crosses, Testing Progeny, and Isolating Phenotypic Assortants. P. Bruns and D. Cassidy-Hanley, Methods for Genetic Analysis. S.L. Allen, Isolation of Micronuclear and Macronuclear DNA. E. Orias and E.P. Hamilton, Genetically Sorting a Collection of Tetrahymena Mutants. E.P. Hamilton and E. Orias, Genetically Mapping New Mutants and Cloned Genes. D.G. Pennock, Selection of Motility Mutants. E.S. Cole and K.R. Stuart, Nuclear and Cytoskeletal Fluorescence Microscopy Techniques. E.S. Cole and K.R. Stuart, Nuclear and Cortical Histology for Brightfield Microscopy. W. Dentler, Fixation of Tetrahymena Cells for Electron Microscopy. R.H. Gavin, J.G. Hoey, and J.A. Garces, Immuno-electron Microscopy of Tetrahymena. A.P. Turkewitz, N.D. Chilcoat, A. Haddad, and J.W. Verbsky, Regulated Protein Secretion in Tetrahymena Thermophila. T.M. Hennessey and H.G. Kuruvilla, Electrophysiology of Tetrahymena. E.A. Wiley, C.A. Mizzen, and C.D. Allis, Isolation and Characterization of in vivo Modified Histones and Activity Gel Assay for Identification of Histone Acetyltransferase. C. Autexier, Tetrahymena Telomerase Activity, Purification, and Reconstruction. E. Blackburn, D. Gilley, T. Ware, A. Bhattacharyya, K. Kirk, and H. Wang, Study the Telomerase RNA in Tetrahymena. T.M. Gibson and D.J. Asai, Isolation and Characterization of 22S Outer Arm Dynein from Tetrahymena Cilia. N.E. Williams, Preparation of Cytoskeletal Fractions from Tetrahymena. N.E. Williams, Immunoprecipitation Procedures. O. Numato, K. Hanyu, T. Takeda, and Y. Watanabe, Tetrahymena Calcium-binding Proteins, TCBP-23. M.-C. Yao, D.L. Chalker, C. Randolph, and J.G. Ward, Microinjection of Tetrahymena Thermophila. J. Gaertig and G. Kapler, Transient and Stable DNA Transformation of Tetrahymena Thermophila by Electroporation. P. Bruns and D. Cassidy-Hanley, Biolistic Transformation of Macro- and Micronuclei. M.A. Gorovsky, B. Hai, and J. Gaertig, Knockout Heterokaryons Enable Facile Mutagenic Analysis of Essential Genes in Tetrahymena. M.-C. Yao, Q. Fan, and R. Sweeney, Creation and Use of Antisense Ribosomes in Tetrahymena Thermophila. M.A. Gorovsky and L. Yu, Protein Tagging in Tetrahymena. S.L. Allen, Appendix I: Genetic Nomenclature Rules for Tetrahymena Thermophila. J. Wuitschick and K. Karrer, Appendix II: Codon Usage in Tetrahymena Thermophila.
- 巻冊次
-
: hb. ISBN 9780125441643
内容説明
Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena as the experimental system of choice.
目次
Overview:
D.L. Nanney and E.M. Simon, Laboratory and Evolutionary History of Tetrahymena thermophila.
J. Frankel, Cell Biology of Tetrahymena thermophila.
K.M. Karrer,Tetrahymena Genetics: Two Nuclei are Better Than One.
Methods:
E. Orias, E.P. Hamilton, and J.D. Orias, Tetrahymena as a Laboratory Organism: Useful Strains, Cell Culture, and Cell Line Maintenance.
P.J. Bruns, H.R. Smith, and D. Cassidy-Hanley, Long-term Storage.
E.P. Hamilton and E. Orias, Genetic Crosses: Setting up Crosses, Testing Progeny, and Isolating Phenotypic Assortants.
P.J. Bruns and D. Cassidy-Hanley, Methods for Genetic Analysis.
S.L. Allen, Isolation of Micronuclear and Macronuclear DNA.
E. Orias and E.P. Hamilton, Genetically Sorting a Collection of Tetrahymena Mutants.
E.P. Hamilton and E. Orias, Genetically Mapping New Mutants and Cloned Genes.
D.G. Pennock, Selection of Motility Mutants.
K.R. Stuart and E.S. Cole, Nuclear and Cytoskeletal Fluorescence Microscopy Techniques.
E.S. Cole and K.R. Stuart, Nuclear and Cortical Histology for Brightfield Microscopy.
W. Dentler, Fixation of Tetrahymena Cells for Electron Microscopy.
R.H. Gavin, J.G. Hoey, and J.A. Garces, Immunoelectron Microscopy of Tetrahymena.
Cell Biology:
A.P. Turkewitz, N.D. Chilcoat, A. Haddad, and J.W. Verbsky, Regulated Protein Secretion in Tetrahymena thermophila.
T.M. Hennessey and H.G. Kuruvilla, Electrophysiology of Tetrahymena.
E.A. Wiley, C.A. Mizzen, and C.D. Allis, Isolation and Characterization of in Vivo Modified Histones and an Activity Gel Assay for Identification of Histone Acetyltransferase.
C. Autexier, Tetrahymena Telomerase Activity, Purification, and Reconstruction.
E. Blackburn, D. Gilley, T. Ware, A. Bhattacharyya, K. Kirk, and H. Wang, Studying the Telomerase RNA in Tetrahymena.
T.M. Gibson and D.J. Asai, Isolation and Characterization of 22S Outer Arm Dynein from Tetrahymena Cilia.
N.E. Williams Preparation of Cytoskeletal Fractions from Tetrahymena thermophila.
N.E. Williams, Immunoprecipitation Procedures.
O. Numato, K. Hanyu, T. Takeda, and Y. Watanabe, Tetrahymena Calcium-Binding Proteins, TCBP-25 and TCBP-23.
Manipulating Genes:
D.L. Chalker, J.G. Ward, C. Randolph, and M.-C. Yao, Microinjection of Tetrahymena thermophila.
J. Gaertig and G. Kapler, Transient and Stable DNA Transformation of Tetrahymena thermophila by Electroporation.
P.J. Bruns and D. Cassidy-Hanley, Biolistic Transformation of Macro- and Micronuclei.
B. Hai, J. Gaertig, an M.A. Gorovsky, Knockout Heterokaryons Enable Facile Mutagenic Analysis of Essential Genes in Tetrahymena.
Q. Fan, R. Sweeney, and M.-C. Yao, Creation and Use of Antisense Ribosomes in Tetrahymena thermophila.
L. Yu and M.A. Gorovsky, Protein Tagging in Tetrahymena.
Appendix I: Genetic Nomenclature Rules for Tetrahymena thermophila.
Appendix II: Codon Usage in Tetrahymena thermophila.
Chapter References.
Index.
Volumes in Series.
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