WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction

書誌事項

WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction

Published on behalf of the World Health Organization by Cambridge University Press, 1999

4th ed

  • pbk.

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注記

Includes bibliographical references (p. 118-124) and index

内容説明・目次

内容説明

This definitive and essential source of reference has been thoroughly up-dated and revised to meet the requirements of all laboratories involved in the analysis of human semen. The book sets out the fundamental laboratory techniques that should be employed in the diagnosis of male infertility. The text includes descriptions of how to construct a conventional semen profile and provides standardized protocols for performing several optional diagnostic procedures. Such techniques are essential in the evaluation of infertile couples and in assessing fertility in men whose sperm production is suppressed by potential anti-fertility compounds or by toxic agents: they are also of interest in forensic medicine and in connection with artificial insemination. Previous editions of this volume have established themselves as the gold standard in the area of fertility investigation and treatment: this new edition continues that tradition and will be the benchmark for setting more rigorous standards for future years.

目次

  • Acknowledgements
  • Abbreviations
  • 1. Introduction
  • 2. Collection and examination of human semen
  • 2A. Standard procedures
  • 2B. Optional tests
  • 2C. Research tests
  • 3. Sperm preparation techniques
  • 4. Quality control in the andrology laboratory
  • 5. Sperm-cervical mucus interactions
  • Appendices. IA. Reference values of semen variables
  • IB. Nomenclature for some semen variables
  • II. Safety guidelines for the andrology laboratory
  • III. Methods for detecting leucocytes
  • IV. Sperm vitality techniques
  • V. Papanicolau staining procedures modified for spermatozoa
  • VI. Shorr staining procedure for sperm morphology
  • VII. Rapid staining procedure for sperm morphology
  • VIII. Immunobead test
  • IX. Mixed antiglobulin reaction test (MAR test)
  • X. Measurement of zinc in seminal plasma
  • XI. Measurement of fructose in seminal plasma
  • XII. Measurement of neutral a-glucosidase in seminal plasma
  • XIII. Sample instructions and record form for semen analysis
  • XIV. Computer-aided sperm analysis (CASA)
  • XV. Protocols for the zona-free hamster test
  • XVI. Measurement of reactive oxygen species generated by sperm suspensions
  • XVII. Induced acrosome reaction assay
  • XVIII. Sperm preparation techniques
  • XIX. Quality control (QC) procedures
  • XX. Sample record form for cervical mucus scoring and postcoital test
  • XXI. Sperm-cervical mucus interaction: the capillary tube test
  • XXII. Basic requirements for an andrology laboratory
  • Bibliography
  • Index.

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