WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction
Author(s)
Bibliographic Information
WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction
Published on behalf of the World Health Organization by Cambridge University Press, 1999
4th ed
- pbk.
Available at 8 libraries
  Aomori
  Iwate
  Miyagi
  Akita
  Yamagata
  Fukushima
  Ibaraki
  Tochigi
  Gunma
  Saitama
  Chiba
  Tokyo
  Kanagawa
  Niigata
  Toyama
  Ishikawa
  Fukui
  Yamanashi
  Nagano
  Gifu
  Shizuoka
  Aichi
  Mie
  Shiga
  Kyoto
  Osaka
  Hyogo
  Nara
  Wakayama
  Tottori
  Shimane
  Okayama
  Hiroshima
  Yamaguchi
  Tokushima
  Kagawa
  Ehime
  Kochi
  Fukuoka
  Saga
  Nagasaki
  Kumamoto
  Oita
  Miyazaki
  Kagoshima
  Okinawa
  Korea
  China
  Thailand
  United Kingdom
  Germany
  Switzerland
  France
  Belgium
  Netherlands
  Sweden
  Norway
  United States of America
Note
Includes bibliographical references (p. 118-124) and index
Description and Table of Contents
Description
This definitive and essential source of reference has been thoroughly up-dated and revised to meet the requirements of all laboratories involved in the analysis of human semen. The book sets out the fundamental laboratory techniques that should be employed in the diagnosis of male infertility. The text includes descriptions of how to construct a conventional semen profile and provides standardized protocols for performing several optional diagnostic procedures. Such techniques are essential in the evaluation of infertile couples and in assessing fertility in men whose sperm production is suppressed by potential anti-fertility compounds or by toxic agents: they are also of interest in forensic medicine and in connection with artificial insemination. Previous editions of this volume have established themselves as the gold standard in the area of fertility investigation and treatment: this new edition continues that tradition and will be the benchmark for setting more rigorous standards for future years.
Table of Contents
- Acknowledgements
- Abbreviations
- 1. Introduction
- 2. Collection and examination of human semen
- 2A. Standard procedures
- 2B. Optional tests
- 2C. Research tests
- 3. Sperm preparation techniques
- 4. Quality control in the andrology laboratory
- 5. Sperm-cervical mucus interactions
- Appendices. IA. Reference values of semen variables
- IB. Nomenclature for some semen variables
- II. Safety guidelines for the andrology laboratory
- III. Methods for detecting leucocytes
- IV. Sperm vitality techniques
- V. Papanicolau staining procedures modified for spermatozoa
- VI. Shorr staining procedure for sperm morphology
- VII. Rapid staining procedure for sperm morphology
- VIII. Immunobead test
- IX. Mixed antiglobulin reaction test (MAR test)
- X. Measurement of zinc in seminal plasma
- XI. Measurement of fructose in seminal plasma
- XII. Measurement of neutral a-glucosidase in seminal plasma
- XIII. Sample instructions and record form for semen analysis
- XIV. Computer-aided sperm analysis (CASA)
- XV. Protocols for the zona-free hamster test
- XVI. Measurement of reactive oxygen species generated by sperm suspensions
- XVII. Induced acrosome reaction assay
- XVIII. Sperm preparation techniques
- XIX. Quality control (QC) procedures
- XX. Sample record form for cervical mucus scoring and postcoital test
- XXI. Sperm-cervical mucus interaction: the capillary tube test
- XXII. Basic requirements for an andrology laboratory
- Bibliography
- Index.
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