Cytotoxic drug resistance mechanisms

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書誌事項

Cytotoxic drug resistance mechanisms

edited by Robert Brown and Uta Böger-Brown

（Methods in molecular medicine / John M. Walker, series editor, 28）

Humana Press, c1999

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

There is now a range of cytotoxic drugs that have considerable clinical usefulness in producing responses in tumors and even, in a small proportion of cases, cure. However, the acquisition of drug resistance is a major clinical problem and is perhaps the main limiting factor in successful treatment of cancer. Thus, a tumor initially sensitive to chemotherapy will, in the majority of cases, eventually recur as a resistant tumor, which will then progress. Much of our understanding of drug resistance mechanisms comes from the study of tumor cell lines grown in tissue culture. We now understand many of the - lecular mechanisms that can lead to a cell acquiring resistance to antic- cer drugs; however, we still do not know which mechanism(s) are those most relevant to the problem of clinical drug resistance. Indeed, given that many of the cytotoxic anticancer drugs were discovered by random screening, it is - clear what features give a clinically useful anticancer drug a sufficient the- peutic index to be of value. The aim of Cytotoxic Drug Resistance Mechanisms is to provide pro- cols that are appropriate for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumor samples. Tumor cell lines have been enormously useful as experimental models of drug resistance mechanisms, however they have limitations and we need to address the relevance of such mechanisms in patients' tumors. Examining drug resistance in tumors is much more problematic than in cell lines.

目次

Drug Resistance: The Clinical Perspective D. Alan Anthoney and Stanley B. Kaye Cell Sensitivity Assays: Clonogenic Assay Jane A. Plumb Cell Sensitivity Assays: The MTT Assay Jane A. Plumb Cell Sensitivity Assays: Detection of Apoptotic Cells In Vitro Using the TUNEL Assay Neil A. Jones and Caroline Dive Analysis of Apoptosis in Tissue Sections Vicki Save, Philip J. Coates, and Peter A. Hall Measurement of P-glycoprotein Function Henk J. Broxterman Measuring MDR-1 by Quantitative RT-PCR Susan E. Bates, Zhirong Zhan, Joanna Regis, and Erick Gamelin Microtiter Plate Technique for the Measurement of Glutathione in Fresh and Cryopreserved Lymphoblasts Using the Enzyme Recycling Method Pamela R. Kearns and Andrew G. Hall Measurement of Reduced Glutathione Using High Pressure Liquid Chromatography Linda A. Hogarth, Celia M. A. Rabello, and Andrew G. Hall Topoisomerase I and II Activity Assays Philippe Pourquier, Glenda Kohlhagen, Li-Ming Ueng, and Yves Pommier 5-Fluorouracil Metabolizing Enzymes Howard L. McLeod, Lesley H. Milne, and Stephen J. Johnston Measuring DNA Adducts by Immunoassay (ELISA) Michael J. Tilby Measuring Drug-DNA Adducts in Individual Cells Adrian J. Frank Measurement of Drug-Induced DNA Interstrand Crosslinking Using the Single Cell Electrophoresis (Comet) Assay Victoria J. Spanswick, Janet M. Hartley, Timothy H. Ward, and John A. Hartley PCR Analysis of Microsatellite Instability Gillian L. Hirst O 6-Alkylguanine-DNA Alkyltransferase Assay Amanda J. Watson and Geoffrey P. Margison Analysis of the p53 Status of Tumors: An Overview of Methods Jonas Bergh Bcl-2 Family Immunohistochemistry Lloyd R. Kelland and Philip J. Beale Genetic Analysis of Drug Resistance by Fluorescence In Situ Hybridization W. Nicol Keith Genetic Analysis of Drug Resistance by ReverseIn Situ Hybridization W. Nicol Keith Index

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