Manual of immunological methods
Author(s)
Bibliographic Information
Manual of immunological methods
（Pharmacology & toxicology）
CRC Press, c1999
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Note
Includes bibliographical references and index
alk. paper
Description and Table of Contents
Description
The Manual of Immunological Methods represents the collaboration of the Canadian Network of Toxicology Centers, a non-profit network of university-based scientists dedicated to research, training, risk assessment, and communication. This manual provides detailed immunological methods that can be utilized by researchers or practitioners who want to enhance the successful application of this science. It emphasizes the need for continuously improving the quality of experiments performed and maintaining consistency in the results obtained.
The Manual of Immunological Methods presents validated assays that are accompanied by a series of tools that will guide readers in performing their own laboratory work in the spirit of the Good Laboratory Practices. It covers specific methodologies including sample collection, preparation of cells, assay conditions, and data evaluation. Discussion of species differences and comparisons of a wide variety of species is an important feature. Both graduate students and practicing researchers in the areas of immunology and immunotoxicology will appreciate the Manual of Immunological Methods as a guide to standard operating procedures in the laboratory.
Table of Contents
Identification, Anaesthesia, and Euthanasia. Collection of Peripheral Blood Samples. Removal of Organs. Preparation of Cell Suspensions. Assessment of Cell Viability. Cell Cryopreservation. Oxidative Burst Assay Using Flow Cytometry. Cell Cytotoxicity. Lymphoblastic Transformation. Determination of Antibody-Producing Cells to a Specific Antigen. Mixed Lymphocyte Reaction (MLR). Intracellular Levels of Calcium Assay Using Flow Cytometry. Phenotyping of Blood Mononuclear Cells. Evaluation of Intracellular Level of Thiols. Apoptosis.
OTIs
Atlas of Immunology, Julius M. Cruse and Robert E. Lewis, University of Mississippi Medical Center, Jackson, Catalog no. 9489, January 1998, c. 350 pp., 7x10, ISBN: 0-8493-489-9, $69.95
Illustrated Dictionary of Immunology, Julius M. Cruse and Robert E. Lewis, Jr., University of Mississippi, Jackson, Catalog no. 4557, 1995, 352 pp., 8.5x11, ISBN: 0-8493-4557-X, $75.00
Handbook of Human Immunology, Mary S. Leffell, Noel R. Rose, and Albert D. Donnenberg, Catalog no. 134, April 1997, 656 pp., 6x9, ISBN: 0-8493-0134-3, $99.95
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