Protein phosphorylation : a practical approach

著者

書誌事項

Protein phosphorylation : a practical approach

edited by D.G. Hardie

（The practical approach series, 211）

Oxford University Press, c1999

2nd ed

  • : hbk
  • : pbk

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.

目次

  • Preface
  • 1. Study of protein phosphorylation in intact cells
  • 2. Analysis of signal transduction pathways using protein kinase inhibitors and activators
  • 3. Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors
  • 4. Analysis of signal transduction pathways using molecular biological manipulations
  • 5. Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates
  • 6. Phosphorylation site analysis by mass spectrometry
  • 7. Assay and purification of protein (serine/threonine) phosphatases
  • 8. [chapter title missing]
  • 9. Identification of substrates for protein-tyrosine phosphatases
  • 10. Assay and purification of protein-serine/threonine kinases
  • 11. Assay of protein kinases and phosphatases using specific peptide substrates
  • 12. Cloning and expression of cDNAs encoding protein kinase subunits
  • 13. Interaction cloning of protein kinase partners
  • 14. Identifying protein kinase substrates by expression screening with solid-phase phosphorylation
  • 15. Analysis of protein kinase interactions using the two-hybrid method
  • 16. Analysis of protein kinase interactions using biomolecular interaction analysis
  • 17. Analysis of protein kinase specificity using orientated peptide libraries
  • 18. Crystallization of protein kinases and phosphatases
  • Index

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