Animal cell biotechnology : methods and protocols
著者
書誌事項
Animal cell biotechnology : methods and protocols
（Methods in biotechnology, 8）
Humana Press, c1999
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.
目次
Immortalization Strategies for Mammalian Cells. Culturing Animal Cells in Fluidized Bed Reactors. Viral Evaluation of Animal Cell Lines Used In Biotechnology. Monitoring Animal Cell Growth and Productivity by Flow Cytometry. Adaptation of Mammalian Cells Grown in Serum Free Medium.Cytotoxicity Testing Using Cell Lines. Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells.Hematopoietic Cells for Cellular and Gene Therapy: I. Basic Assay Techniques. Measurement of Cell-Culture Glycosidase Activity. Cytogenetic Characterization of Recombinant Cells. Hematopoietic Cells for Cellular and Gene Therapy: II. Expansion Protocols.Control of Proteolysis in Cell Culture. Measurement of Cell Death in Culture. GPI-Anchored Fusion Proteins. Harvesting GPI-Anchored Proteins From CHO Cells. Optimizing Gene Expression in Mammalian Cells.
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