Protein localization by fluorescent microscopy : a practical approach

著者

    • Allan, Victoria J.

書誌事項

Protein localization by fluorescent microscopy : a practical approach

edited by Victoria J. Allan

（The practical approach series, 218）

Oxford University Press, c2000

  • :hbk
  • :pbk

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user.

目次

  • Preface
  • 1. Basic Immunofluorescence
  • 2. Immunofluorescent Labelling of Sections
  • 4. Instruments for Fluorescence Imaging
  • 5. Fluorescence Microscopy of Living Vertebrate Cells
  • 6. Visualising Fluorescence in Drosophila - optimal detection in thick specimens
  • 7. Green Fluorescent Protein in Plants
  • 8. Fluorescence Microscopy in Yeast
  • Index

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