Cell growth, differentiation and senescence : a practical approach
著者
書誌事項
Cell growth, differentiation and senescence : a practical approach
(The practical approach series, 215)
Oxford University Press, c1999
- : hbk
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199637683
内容説明
There are three main themes running through this volume. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but in vivo procedures are also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme
presented here is growth cessation, illustrated by several systems for induction of cell differentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Importantly, well proved methods for
studying cell growth are supplemented by some novel approaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. Only two chapters have been retained, in an updated form from Cell Growth and Apoptosis, the predecessor volume. The book is written by a team of scientists highly experienced in procedures they describe, and offer details and hints found valuable in their own laboratories; thus, variants of the same general methods can be found in
different chapters. These should be helpful to beginning as well as experienced investigators, and are designed to stimulate new approaches to old and new questions.
目次
- PREFACE
- CHAPTER 1 WIEDER: SELECTION OF METHODS FOR MEASURING PROLIFERATION
- CHAPTER 2 SKEHAN: CELL GROWTH AND CYTOTOXICITY ASSAYS
- CHAPTER 3 DURAND: CELL GROWTH AND KINETICS IN MULTICELL SPHEROIDS
- CHAPTER 4 O'CONNOR & JACKMAN: ASSESSMENT OF CHECKPOINT CONTROLS OF CELL GROWTH
- CHAPTER 5 BISHAYEE: ASSESSMENT OF THE ROLE OF GROWTH FACTORS AND THEIR RECEPTORS IN CELL PROLIFERATION
- CHAPTER 6 ALESSANDRINI: ANALYSIS OF GROWTH-SIGNALING PROTEIN KINASE CASCADES
- CHAPTER 7 JUAN ET AL: CYTOMETRIC ANALYSIS OF THE PRB PATHWAY
- CHAPTER 8 COSENZA ET AL: METHODS FOR ONCOGENE DETECTION
- CHAPTER 9 STEIN ET AL: ISOLATION AND VISUALIZATION OF THE PRINCIPAL COMPONENTS OF NUCLEAR ARCHITECTURE
- CHAPTER 10 WOLF & FLANAGAN: ANTISENSE OLIGONUCLEOTIDES - TOOLS FOR ELUCIDATING GENE FUNCTION
- CHAPTER 11 MOORE & STUDZINSKI: HUMAN LEUKEMIA CELLS AS A MODEL DIFFERENTIATION SYSTEM
- CHAPTER 12 CROSS & KALLEY: INDUCTION OF DIFFERENTIATION OF HUMAN INTESTINAL CELL IN VITRO
- CHAPTER 13 THALHAMMER & CROSS: PROLIFERATION... [DATA INCOMPLETE]
- 巻冊次
-
: hbk ISBN 9780199637690
内容説明
There are three main themes running through this volume. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but in vivo procedures are also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme
presented here is growth cessation, illustrated by several systems for induction of cell differentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Importantly, well proved methods for
studying cell growth are supplemented by some novel approaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. Only two chapters have been retained, in an updated form from Cell Growth and Apoptosis, the predecessor volume. The book is written by a team of scientists highly experienced in procedures they describe, and offer details and hints found valuable in their own laboratories; thus, variants of the same general methods can be found in
different chapters. These should be helpful to beginning as well as experienced investigators, and are designed to stimulate new approaches to old and new questions.
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