Lymphocytes : a practical approach
著者
書誌事項
Lymphocytes : a practical approach
（The practical approach series, 220）
Oxford University Press, c2000
2nd ed
- :hbk.
- :pbk.
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注記
Previous ed.: edited by G. G. B. Klaus. 1987
内容説明・目次
内容説明
Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the
generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification
assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.
目次
- Preface
- Preparation of lymphocytes and idenfication of lymphocyte subpopulations
- Immunohistochemistry of lymphoid organs
- T and B-cell hybridomas
- Murine T-cell culture
- Human CD4+ T-cell culture
- Human Cytotoxic T-cell culture
- Limiting dilution analysis for quantification of antigen-specific T-cells
- HLA -peptide tetrameric complexes
- Expansion of human T-cells for immunotheraphy
- HLA typing
- Characterisation of lymphocyte surface markers
- Apoptosis assays for lymphocytes
- Thymic organ culture
- Index
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