Apoptosis : a practical approach
著者
書誌事項
Apoptosis : a practical approach
（The practical approach series, 216）
Oxford University Press, c1999
- :pbk.
- :hbk.
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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:hbk. ISBN 9780199637843
内容説明
This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis, as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research.
目次
- Studzinski - overview of apoptosis
- Wilson and Potten - the morphological recognition of apoptosis
- Yoshida, Shao and Pommier - assessment of DNA damage in apoptosis
- Darzynkiewicz, Bedner and Li - analysis of cell death by flow and laser scanning cytometry
- Naval and Anel - cell-mediated cytotoxicity and cell death receptors
- Jenkins and Hannun - sphingolipids as messengers of cell death
- Van Engeland et al - cytochemical detection of cyto- and nucleoskeletal changes during apoptosis
- Costa-Pereira and Cotter
- Reed et al - methods of measuring Bcl-2 family proteins and their functions
- Blajeski and Kaufmann - methods for detecting proteolysis during apoptosis in intact cells.
- 巻冊次
-
:pbk. ISBN 9780199637850
内容説明
This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for
apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research.
目次
- Preface
- 1. Overview of Apoptosis
- 2. The Morphological Recognition of Apoptosis
- 3. Assessment of DNA Damage in Apoptosis
- 4. Analysis of Cell Death by Flow and Laser Scanning Cytometry
- 5. Cell-mediated Cytotoxicity and Cell Death Receptors
- 6. Sphingolipids as Messengers of Cell Death
- 7. Cytochemical Detection of Cyto- and Nucleoskeletal Changes During Apoptosis
- 9. Methods of Measuring Bcl-2 Family Proteins and Their Functions
- 10. Methods for Detecting Proteolysis During Apoptosis in Intact Cells
- Index
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