Mycoplasma protocols

著者

• Miles, Roger (Roger J.)
• Nicholas, Robin

書誌事項

Mycoplasma protocols

edited by Roger Miles and Robin Nicholas

（Methods in molecular biology / John M. Walker, series editor, v. 104）

Humana Press, 1998

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides SC, in Europe and Africa and the discovery of a possible association between mycoplasmas and AIDS. During this period there has also been a recognition by control authorities and int- national organizations, including the Office International des Epizooties, that mycoplasmas are major causes of economic loss in livestock and continue to be a problem in humans as a result of a range of chronic respiratory and urogenital diseases. However, it is likely that the true significance of my- plasmas is still underestimated because of the difficulties in working with these extremely fastidious microorganisms. Advances in such laboratory technology as DNA amplification has enabled the detection of unrecoverable mycoplasmas in tissue samples and this will provide us with a better understanding of their role in disease. Important developments have also been seen in more conv- tional areas such as serological tests and media formulation enabling improved recovery and identification. Mycoplasma Protocols provides in a single volume up-to-date and easy-- follow methods for the detection, isolation, identification, and characterization of mycoplasmas, with a major emphasis on those of medical and veterinary significance. It also includes biochemical, genetic, and molecular techniques that will form the basis of understanding pathogenicity and, particularly, - hesion to host cells.

目次

Introduction R. Miles and R. Nicholas The Medical Significance of Mycoplasmas P. Taylor The Veterinary Significance of Mycoplasmas R. Nicholas Recovery of Mycoplasmas from Man P. Taylor Recovery of Mycoplamas from Animals R. Nicholas and S. Baker Recovery of Mycoplasmas from Birds J. Bradbury Cultivation of Ureaplasmas P. Furr Quality Control Testing of Mycoplasma Media D. Windsor and H. Windsor Biochemical Characteristics of Mycoplasma Identification J. Poveda Enzyme Analysis: The Rationale and Use of Enzyme Assays in Assigning Function to Gene Nucleotide Sequences and the Procedures for the Assay of Three Enzymatic Functions Conserved in All Mollicutes J. D. Pollack Determination of Substrate Utilization Rates by Mycoplasmas R. Miles and C. Agbanyim Serological Identification of Mycoplasmas by Growth and Metabolism Inhibition Tests J. Poveda and R. Nicholas Identification of Mycoplasmas by Dot Immunobinding on Membrane Filtration (MF dot) F. Poumarat Identification of Mycoplasmas by Immunofluorescence J. Bradbury Diagnostic Application of Monoclonal Antibody-Based Sandwich ELISAs H. Ball and D. Finlay Immunocytochemical staining of Fixed Tissues E. Scanziani Extraction of DNA from Mycoplasmas J. Bashiruddin Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers K. E. Johansson, M. Heltander, and B. Petterson PCR and RFLP Methods for Specific Detection and Identification of Mycoplasma Mycoides Subsp. Mycoides SC J. Bashiruddin Characterization of Mycoplasmas by RAPD Fingerprinting G. Rawadi Classification of Isolates by DNA-DNA Hybridization K. Sachse and H. Hotzel. Insertion Sequence Analysis J. Frey Detection of Mycoplasmas in Cell Cultures by Cultural Methods G. Masover and F. Becker Detection of Mycoplasmas in Cell Culture by Fluorescence Methods G. Masover and F. Becker Transformation of Mycoplasmas F. Chris Minion, and P. Kapke Transposon Mutagenesis L. Voelker and K. Dybvig Demonstration of Extrachromosomal Elements L. Voelker and K. Dybvig Expression of Foreign Genes in Acholeplasma T. Jarhede and A. Wieslander Mycoplasma Gene Expression in Escherichia Coli F. C. Minion Polyacrylamide Gel Electrophoresis Separation of Whole Cell Proteins M. Duffy, A. Noormohammadi, N. Baseggio, G. Browning, and P. Markham Immunological and Biochemical Characterization of Membrane Proteins M. Duffy A. Noormohammadi N. Baseggio, G. Browning, and P. Markham Detection and Analysis of Mycoplasma Adhesins K. Sachse Transmission Electron Microscopy and Immunogold Staining of Mycoplasma Surface Antigens G. Christiansen and S. Birkelund Index.

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詳細情報

• NII書誌ID(NCID): BA45474249
• ISBN:
  • 0896035255
• LCCN: 98004288
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xiii, 330 p.
• 大きさ: 24 cm
• 分類:
  • LCC : QR352
  • DC21 : 579.3/28
• 件名:
  • LCSH : Mycoplasma -- Laboratory manuals
  • LCSH : Mycoplasma diseases -- Laboratory manuals
• 親書誌ID:
  • BA10794673