Transcription factor protocols

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書誌事項

Transcription factor protocols

edited by Martin J. Tymms

（Methods in molecular biology / John M. Walker, series editor, v. 130）

Humana Press, c2000

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The effort to sequence the human genome is now moving toward a c- clusion. As all of the protein coding sequences are described, an increasing emphasis will be placed on understanding gene function and regulation. One important aspect of this analysis is the study of how transcription factors re- late transcriptional initiation by RNA polymerase II, which is responsible for transcribing nuclear genes encoding messenger RNAs. The initiation of Class II transcription is dependent upon transcription factors binding to DNA e- ments that include the core or basal promoter elements, proximal promoter elements, and distal enhancer elements. General initiation factors are involved in positioning RNA polymerase II on the core promoter, but the complex - teraction of these proteins and transcriptional activators binding to DNA e- ments outside the core promoter regulate the rate of transcriptional initiation. This initiation process appears to be a crucial step in the modulation of mRNA levels in response to developmental and environmental signals. Transcription Factor Protocols provides step-by-step procedures for key techniques that have been developed to study DNA sequences and the protein factors that regulate the transcription of protein encoding genes. This volume is aimed at providing researchers in the field with the well-detailed protocols that have been the hallmark of previous volumes of the Methods in Molecular (TM) Biology series.

目次

Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method Dennis K. Watson, Richard Kitching, Calvin Vary, Ismail Kola, and Arun Seth In Vivo Footprinting Using UV Light and Ligation-Mediated PCR Gerd P. Pfeifer and Stella Tommasi Identification of DNaseI Hypersensitive Sites Within Nuclei Peter N. Cockerill Analysis of In Vivo Methylation Hans-Henrik M. Dahl and Wendy M. Hutchison Detection of Transcription Factor Partners with a Yeast One Hybrid Screen Michael Sieweke Inverse PCR (IPCR) for Obtaining Promoter Sequence Tony Triglia PCR-Directed Linker Scanning Mutagenesis Kurt Gustin and Robert D. Burk Transfection Technologies Elaine T. Schenborn The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues Charles M. Nicolet and Ning-Sun Yang Optimizing Electroporation Conditions for the Transformation of Mammalian Cells William C. Heiser Calcium Phosphate Transfection of Mammalian Cultured Cells Elaine T. Schenborn and Virginia Goiffon DEAE-Dextran Transfection of Mammalian Cultured Cells Elaine T. Schenborn and Virginia Goiffon Liposome-Mediated Transfection of Mammalian Cells Elaine T. Schenborn and Jennifer Oler Assays for Transcriptional Activity Based on the Luciferase Reporter Gene S. Roy Himes and M. Frances Shannon Transient Transfection of Schneider Cells in the Study of Transcription Factors Guntram Suske Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription Marina Kochetkova and M. Frances Shannon Expression and Purification of Histidine-Tagged Transcription Factors Donna R. Cohen Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein Ulrich Kruse, Thorsten T. Ebel, and Albrecht E. Sippel Electrophoretic Mobility Shift Assays Peter L. Molloy In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors Martin J. Tymms In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors Fujiko Watt Computer Software of Eukaryotic Promoter Analysis Dan S. Prestridge Index

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