CiNii 図書 - Developmental biology protocols

Developmental biology is one of the most exciting and fast-growing fields - day. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences alike. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Part I. Introduction. Developmental Biology Protocols: Overview I, Rocky S. Tuan and Cecilia W. Lo. Part II. Systems: Production, Culture, and Storage. Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies, Christopher J. Lowe and Gregory A. Wray. Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins, James A. Coffman and Patrick S. Leahy. The Chick Embryo as a Model System for Analyzing Mechanisms of Development, Diana K. Darnell and Gary C. Schoenwolf. Culture of Avian Embryos, Diana K. Darnell and Gary C. Schoenwolf. Ex Ovo Culture of Avian Embryos, Tamao Ono. Culture of Preimplantation Mouse Embryos, Adam S. Doherty and Richard M. Schultz. In Vitro Culture of Rodent Embryos During Early Postimplantation Period, Masahiko Fujinaga. Cryopreservation of Mouse Embryos, Jean Richa. Part III. Developmental Pattern and Morphogenesis. Studying Head and Brain Development in Drosophila, Robert Finkelstein. Bioassays of Inductive Interactions in Amphibian Development, Takashi Ariizumi, Kazuhiro Takano, Makoto Asashima, and George M. Malacinski. Gastrulation and Early Mesodermal Patterning in Vertebrates, Gary C. Schoenwolf and Jodi L. Smith. Craniofacial Development of Avian and Rodent Embryos, Brian K. Hall and Tom Miyake. Examination of the Axial Skeleton of Fetal Rodents, Michael G. Narotsky and John M. Rogers. Cardiac Morphogenesis and Dysmorphogenesis: An Immunohistochemical Approach, B. Rush Waller III and Andy Wessels. Part IV. Embryo Structure and Function. Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos, Kersti K. Linask and Takeshi Tsuda. Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos, Carolyn A. Larabell. Whole-Mount Immunolabeling of Embryos by Microinjection: Increased Detection Levels of Extracellular and Cell Surface Epitopes, Charles D. Little and Christopher J. Drake. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos, Robert M. Zucker, E. Sidney Hunter III, and John M. Rogers. Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy, Robert M. Zucker and John M. Rogers. Magnetic Resonance Imaging Analysis of Embryos, Bradley R. Smith. Optical Coherence Tomography Imaging in Developmental Biology, Stephen A. Boppart, Mark E. Brezinski, and James G. Fujimoto. Ultrasound Backscatter Microscopy of Mouse Embryos, Daniel H. Turnbull. Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function, Kersti K. Linask and James C. Huhta. Calcium Imaging and Cell-Cell Signaling, Diane C. Slusarski and Victor G. Corces. Acquisition, Display, and Analysis of Digital Three-Dimensional and Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications, Charles F. Thomas and John G. White. Part V. Cell Lineage Analysis. Cell Lineage Analysis: Applications of Green Fluorescent Protein, Magdalena Zernicka-Goetz and Jonathon Pines. Cell Lineage Analysis: X-Inactivation Mosaics, Seong-SengTan, Leanne Godinho, and Patrick P. L. Tam. Retroviral Cell Lineage Analysis in the Developing Chick Heart, Robert G. Gourdie, Gang Cheng, Robert P. Thompson, and Takashi Mikawa. Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos, Diana K. Darnell, Virginio Garcia-Martinez, Carmen Lopez-Sanchez, Shipeng Yuan, and Gary C. Schoenwolf. Cell Lineage Analysis: Videomicroscopy Techniques, Paul J. Heid and Jeff Hardin. Cell Lineage Analysis in Xenopus Embryos, Sally A. Moody. Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryos, David J. Kozlowski and Eric S. Weinberg. Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage in the Embryo, Dazhong Sun, C. May Griffith, and Elizabeth D. Hay. Part VI. Chimeras. Transplantation Chimeras: Use in Analyzing Mechanisms

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in "experimental emb- ology" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Part I. Introduction. Developmental Biology Protocols: Overview II, Rocky S. Tuan and Cecilia W. Lo. Part II. Organogenesis. Drosophila as a Genetic Tool to Define Vertebrate Pathway Players, Nancy M. Bonini. Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis, Makoto Asashima, Takashi Ariizumi, Shuji Takahashi, and George M. Malacinski. Analysis of Mammary Gland Morphogenesis, Calvin D. Roskelley, Colleen Wu, and Aruna M. Somasiri. Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro, H. Joseph Yost. Craniofacial Development and Patterning, Harold Slavkin, Glen Nuckolls, and Lillian Shum. Craniofacial Skeletel Morphogenesis In Vitro, Roy C. Ogle. Skeletal Morphogenesis, Stefan Mundlos. Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration, David L. Stocum. Retroviral Infection of T-Cell Precursors in Thymic Organ Culture, Lisa M. Spain, Lisa L. Lau, and Yousuke Takahama. Assay for the Isolation of Hepatogenic Factors: Key Molecules in Hepatocyte Formation, Johannes A. A. Spijkers, Theodorus B. M. Hakvoort, and Wouter H. Lamers. Skin Morphogenesis: Embryonic Chicken Skin Explant Cultures, Cheng-Ming Chuong. Apoptosis in Development, Lin Lin and Zahra F. Zakeri. Methods to Detect Patterns of Cell Death in Drosophila, Nancy M. Bonini. Part III. Abnormal Development and Teratology. Mechanisms of Neurulation, Gary C. Schoenwolf and Jodi L. Smith. Neurulation and Neural Tube Closure Defects, Andrew Copp, Patricia Cogram, Angeleen Fleming, Dianne Gerrelli, Deborah Henderson, Andrew Hynes, Maria Kolatsi-Joannou, Jennifer Murdoch, and Patricia Ybot-Gonzales. Neural Tube Defects, Takayuki Inagaki, Jodi L. Smith, Marion L. Walker, and Gary C. Schoenwolf. Experimental Manipulation and Morphometric Analysis of Neural Tube Development, Mary E. Desmond and Patricia A. Haas. Isolation of Neuroepithelium and Formation of Minispheres, Mary E. Desmond and Marcia C. Field. Examination of Normal and Abnormal Placentation in the Mouse, Michael R. Blackburn. Palatal Dysmorphogenesis: Palate Organ Culture, Barbara D. Abbott. Palatal Dysmorphogenesis: Quantitative RT-PCR, Gary A. Held and Barbara D. Abbott. Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities, Sheila M. Bell, Claire M. Schreiner, and William J. Scott. Assessment of Laterality Defects in Rodent Embryos, Masahiko Fujinaga. Cardiac Morphogenesis and Dysmorphogenesis: I. Normal Development, Andy Wessels and Roger Markwald. Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart: Heart Tube Position and Heart Tube Chirality, Alvin J. Chin. Biologically Based Risk Assessment Models for Developmental Toxicity, Christopher Lau and R. Woodrow Setzer. Part IV. Screening and Mapping of Novel Genes and Mutations. Positional Cloning, Lin Xu and Yefu Li. Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse, Gary E. Lyons, Bradley J. Swanson, Melissa A. Haendel, and Joshua Daniels. PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes, Jian Zhang and Mary Lou King. Analysis of mRNA Expression During Preimplantation Development, Keith E. Latham, Elena De La Casa, and Richard M. Schultz. Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos, Akihiko Shimono and Richard R. Behringer. HPLC-Based mRNA Differential Display, Thomas B. Knudsen. Part V. Transgenesis: Production and Gene Knockout. Production of Transgenic Drosophila, Miki Fujioka, James B. Jaynes, Amy Bejsovec, and Michael Weir. Transgenic Manipulation of the Sea Urchin Embryo, Jonathan P. Rast. Transgenic Zebrafish, Shuo Lin. Production of Avian Chimeras and Germline Transmission, Kristin L. Woods, Scott Schau, Mary Ellen Clark, Jacqueline A. Bonselaar, and Robert J. Etches. Incorporation of Genetically Modified Cells into Chicken Chimeras, Laura D.

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Part I. Introduction. Developmental Biology Protocols: Overview III, Tuan and Lo. Part II. Manipulation of Developmental Gene Expression and Function. Ectopic Expression in Drosophila, Wilder. Clonal Analysis in the Examination of Gene Function in Drosophila, Rooke, Theodosiou, and Xu. Application of Antisense Oligonucleotides in Developing Chicken Embryos, Alexander, Barnes, and Tuan. Application of Functional Blocking Antibodies: N-Cadherin and Chick Embryonic Limb Development, Oberlender and Tuan. Part III. Analysis of Gene Expression. Gene Expression Analyzed by Ribonuclease Protection Assay, Bennett. Relative RT-PCR, Giambernardi and Klebe. Gene Expression Analysis Using Quantitative RT-PCR and a Multispecific Internal Control, Shire and Legoux. In Situ PCR Detection of HIV Expression in the Human Placenta, Sheikh, Polliotti, and Miller. Gene Expression Analysis by In Situ Hybridization: Radioactive Probes, Wawersik and Epstein. Radio-Isotopic In Situ Hybridization on Tissue Sections: Practical Aspects and Quantification, Moorman, De Boer, Hagoort, Franco, and Lamers. mRNA and Protein Colocalization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry, Tuan. Whole-Mount In Situ Hybridization to Study Gene Expression during Mouse Development, Lowe and Kuehn. Multicolor Whole-Mount In Situ Hybridization, Hauptmann and Gerster. Methods for Double Detection of Gene Expression: Combined In Situ Hybridization and Immunohistochemistry or Histochemistry, Conlon. Visualization of the Expression of Green Fluorescent (GFP)-Linked Proteins, Ayoob, Jean Sanger, and Joseph Sanger. Part IV. Models of Morphogenesis and Development. Monoclonal Antibodies in the Analysis of Embryonic Development, Bedian. Mesoderm Induction in Xenopus: Oocyte Expression System and Animal Cap Assay, Yao, Kessler. Amphibian Organizer Activity, Niehrs. Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery, Packard, Cox,and Poole. Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration, Newgreen and Murphy. The Chimeric Human/Mouse Model of Angiogenesis, Petitclerc, von Schalscha, and Brooks. Analysis of Embryonic Vascular Morphogenesis, Sato and Bartunkova. Epithelial-Mesenchyme Interactions, Hall. Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning, Ros, Simandl, Clark, and Fallon. Palate Development--In Vitro Procedures, Pisano and Greene. Part V. In Vitro Models and Analysis Differentiation and Development. In Vitro Fertilization Heyner, Tucker. Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development, Peters, Chapman, and Soares. Bone Marrow-Derived Mesenchymal Progenitor Cells, Johnstone and Yoo. Identification, Characterization, and Differentiation of Human Prostate Cells, Mehta, Perez-Stable, Roos, and Nadji. Preparation of Chick Striated Muscle Cultures, DiLullo, George-Weinstein, and Gerhart. Study of Skeletal Myogenesis in Cultures of Unsegmented Somitic Mesoderm, Borycki and Emerson. Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Chondrocyte Maturation, DeLise, Stringa, Woodward, Mello, and Tuan. Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells, DeLise and Tuan. Murine Cells, Haas and Tuan. Skeletogenesis: In Vitro Analysis of Bone Cell Differentiation, Majolagbe and Robey. Studying Early Hematopoiesis Using Avian Blastoderm Cultures, Eisenberg. Isolation and Culture of Mouse Germ Cells, De Miguel and Donovan. Cadherin-Mediated Cell-Cell Interactions, Knudsen and Soler. Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Progeins, Underhill and Zhang. Microinjection of Fluorescently Labeled Alpha-Actinin into Living Cells, Jean Sanger, Danowski, and Joseph Sanger. Pax-3 and Vertebrate Development, Epstein. Genetic-Engineered Models of Skeletal Diseases I. Collagen Type X, Jacenko.

Developmental biology is one of the most exciting and fast-growing fields - day. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences alike. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Part I. Introduction. Developmental Biology Protocols: Overview I, Rocky S. Tuan and Cecilia W. Lo. Part II. Systems: Production, Culture, and Storage. Rearing Larvae of Sea Urchins and Sea Stars for Developmental Studies, Christopher J. Lowe and Gregory A. Wray. Large-Scale Culture and Preparation of Sea Urchin Embryos for Isolation of Transcriptional Regulatory Proteins, James A. Coffman and Patrick S. Leahy. The Chick Embryo as a Model System for Analyzing Mechanisms of Development, Diana K. Darnell and Gary C. Schoenwolf. Culture of Avian Embryos, Diana K. Darnell and Gary C. Schoenwolf. Ex Ovo Culture of Avian Embryos, Tamao Ono. Culture of Preimplantation Mouse Embryos, Adam S. Doherty and Richard M. Schultz. In Vitro Culture of Rodent Embryos During Early Postimplantation Period, Masahiko Fujinaga. Cryopreservation of Mouse Embryos, Jean Richa. Part III. Developmental Pattern and Morphogenesis. Studying Head and Brain Development in Drosophila, Robert Finkelstein. Bioassays of Inductive Interactions in Amphibian Development, Takashi Ariizumi, Kazuhiro Takano, Makoto Asashima, and George M. Malacinski. Gastrulation and Early Mesodermal Patterning in Vertebrates, Gary C. Schoenwolf and Jodi L. Smith. Craniofacial Development of Avian and Rodent Embryos, Brian K. Hall and Tom Miyake. Examination of the Axial Skeleton of Fetal Rodents, Michael G. Narotsky and John M. Rogers. Cardiac Morphogenesis and Dysmorphogenesis: An Immunohistochemical Approach, B. Rush Waller III and Andy Wessels. Part IV. Embryo Structure and Function. Application of Plastic Embedding for Sectioning Whole-Mount Immunostained Early Vertebrate Embryos, Kersti K. Linask and Takeshi Tsuda. Confocal Microscopy of Live Xenopus Oocytes, Eggs, and Embryos, Carolyn A. Larabell. Whole-Mount Immunolabeling of Embryos by Microinjection: Increased Detection Levels of Extracellular and Cell Surface Epitopes, Charles D. Little and Christopher J. Drake. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos, Robert M. Zucker, E. Sidney Hunter III, and John M. Rogers. Embryo/Fetal Topographical Analysis by Fluorescence Microscopy and Confocal Laser Scanning Microscopy, Robert M. Zucker and John M. Rogers. Magnetic Resonance Imaging Analysis of Embryos, Bradley R. Smith. Optical Coherence Tomography Imaging in Developmental Biology, Stephen A. Boppart, Mark E. Brezinski, and James G. Fujimoto. Ultrasound Backscatter Microscopy of Mouse Embryos, Daniel H. Turnbull. Use of Doppler Echocardiography to Monitor Embryonic Mouse Heart Function, Kersti K. Linask and James C. Huhta. Calcium Imaging and Cell-Cell Signaling, Diane C. Slusarski and Victor G. Corces. Acquisition, Display, and Analysis of Digital Three-Dimensional and Time-Lapse (Four-Dimensional) Data Sets Using Free Software Applications, Charles F. Thomas and John G. White. Part V. Cell Lineage Analysis. Cell Lineage Analysis: Applications of Green Fluorescent Protein, Magdalena Zernicka-Goetz and Jonathon Pines. Cell Lineage Analysis: X-Inactivation Mosaics, Seong-SengTan, Leanne Godinho, and Patrick P. L. Tam. Retroviral Cell Lineage Analysis in the Developing Chick Heart, Robert G. Gourdie, Gang Cheng, Robert P. Thompson, and Takashi Mikawa. Dynamic Labeling Techniques for Fate Mapping, Testing Cell Commitment, and Following Living Cells in Avian Embryos, Diana K. Darnell, Virginio Garcia-Martinez, Carmen Lopez-Sanchez, Shipeng Yuan, and Gary C. Schoenwolf. Cell Lineage Analysis: Videomicroscopy Techniques, Paul J. Heid and Jeff Hardin. Cell Lineage Analysis in Xenopus Embryos, Sally A. Moody. Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryos, David J. Kozlowski and Eric S. Weinberg. Carboxyfluorescein as a Marker at Both Light and Electron Microscope Levels to Follow Cell Lineage in the Embryo, Dazhong Sun, C. May Griffith, and Elizabeth D. Hay. Part VI. Chimeras. Transplantation Chimeras: Use in Analyzing Mechanisms

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in "experimental emb- ology" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Part I. Introduction. Developmental Biology Protocols: Overview II, Rocky S. Tuan and Cecilia W. Lo. Part II. Organogenesis. Drosophila as a Genetic Tool to Define Vertebrate Pathway Players, Nancy M. Bonini. Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis, Makoto Asashima, Takashi Ariizumi, Shuji Takahashi, and George M. Malacinski. Analysis of Mammary Gland Morphogenesis, Calvin D. Roskelley, Colleen Wu, and Aruna M. Somasiri. Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro, H. Joseph Yost. Craniofacial Development and Patterning, Harold Slavkin, Glen Nuckolls, and Lillian Shum. Craniofacial Skeletel Morphogenesis In Vitro, Roy C. Ogle. Skeletal Morphogenesis, Stefan Mundlos. Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration, David L. Stocum. Retroviral Infection of T-Cell Precursors in Thymic Organ Culture, Lisa M. Spain, Lisa L. Lau, and Yousuke Takahama. Assay for the Isolation of Hepatogenic Factors: Key Molecules in Hepatocyte Formation, Johannes A. A. Spijkers, Theodorus B. M. Hakvoort, and Wouter H. Lamers. Skin Morphogenesis: Embryonic Chicken Skin Explant Cultures, Cheng-Ming Chuong. Apoptosis in Development, Lin Lin and Zahra F. Zakeri. Methods to Detect Patterns of Cell Death in Drosophila, Nancy M. Bonini. Part III. Abnormal Development and Teratology. Mechanisms of Neurulation, Gary C. Schoenwolf and Jodi L. Smith. Neurulation and Neural Tube Closure Defects, Andrew Copp, Patricia Cogram, Angeleen Fleming, Dianne Gerrelli, Deborah Henderson, Andrew Hynes, Maria Kolatsi-Joannou, Jennifer Murdoch, and Patricia Ybot-Gonzales. Neural Tube Defects, Takayuki Inagaki, Jodi L. Smith, Marion L. Walker, and Gary C. Schoenwolf. Experimental Manipulation and Morphometric Analysis of Neural Tube Development, Mary E. Desmond and Patricia A. Haas. Isolation of Neuroepithelium and Formation of Minispheres, Mary E. Desmond and Marcia C. Field. Examination of Normal and Abnormal Placentation in the Mouse, Michael R. Blackburn. Palatal Dysmorphogenesis: Palate Organ Culture, Barbara D. Abbott. Palatal Dysmorphogenesis: Quantitative RT-PCR, Gary A. Held and Barbara D. Abbott. Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities, Sheila M. Bell, Claire M. Schreiner, and William J. Scott. Assessment of Laterality Defects in Rodent Embryos, Masahiko Fujinaga. Cardiac Morphogenesis and Dysmorphogenesis: I. Normal Development, Andy Wessels and Roger Markwald. Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart: Heart Tube Position and Heart Tube Chirality, Alvin J. Chin. Biologically Based Risk Assessment Models for Developmental Toxicity, Christopher Lau and R. Woodrow Setzer. Part IV. Screening and Mapping of Novel Genes and Mutations. Positional Cloning, Lin Xu and Yefu Li. Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse, Gary E. Lyons, Bradley J. Swanson, Melissa A. Haendel, and Joshua Daniels. PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes, Jian Zhang and Mary Lou King. Analysis of mRNA Expression During Preimplantation Development, Keith E. Latham, Elena De La Casa, and Richard M. Schultz. Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos, Akihiko Shimono and Richard R. Behringer. HPLC-Based mRNA Differential Display, Thomas B. Knudsen. Part V. Transgenesis: Production and Gene Knockout. Production of Transgenic Drosophila, Miki Fujioka, James B. Jaynes, Amy Bejsovec, and Michael Weir. Transgenic Manipulation of the Sea Urchin Embryo, Jonathan P. Rast. Transgenic Zebrafish, Shuo Lin. Production of Avian Chimeras and Germline Transmission, Kristin L. Woods, Scott Schau, Mary Ellen Clark, Jacqueline A. Bonselaar, and Robert J. Etches. Incorporation of Genetically Modified Cells into Chicken Chimeras, Laura D.

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events-changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Part I. Introduction. Developmental Biology Protocols: Overview III, Tuan and Lo. Part II. Manipulation of Developmental Gene Expression and Function. Ectopic Expression in Drosophila, Wilder. Clonal Analysis in the Examination of Gene Function in Drosophila, Rooke, Theodosiou, and Xu. Application of Antisense Oligonucleotides in Developing Chicken Embryos, Alexander, Barnes, and Tuan. Application of Functional Blocking Antibodies: N-Cadherin and Chick Embryonic Limb Development, Oberlender and Tuan. Part III. Analysis of Gene Expression. Gene Expression Analyzed by Ribonuclease Protection Assay, Bennett. Relative RT-PCR, Giambernardi and Klebe. Gene Expression Analysis Using Quantitative RT-PCR and a Multispecific Internal Control, Shire and Legoux. In Situ PCR Detection of HIV Expression in the Human Placenta, Sheikh, Polliotti, and Miller. Gene Expression Analysis by In Situ Hybridization: Radioactive Probes, Wawersik and Epstein. Radio-Isotopic In Situ Hybridization on Tissue Sections: Practical Aspects and Quantification, Moorman, De Boer, Hagoort, Franco, and Lamers. mRNA and Protein Colocalization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry, Tuan. Whole-Mount In Situ Hybridization to Study Gene Expression during Mouse Development, Lowe and Kuehn. Multicolor Whole-Mount In Situ Hybridization, Hauptmann and Gerster. Methods for Double Detection of Gene Expression: Combined In Situ Hybridization and Immunohistochemistry or Histochemistry, Conlon. Visualization of the Expression of Green Fluorescent (GFP)-Linked Proteins, Ayoob, Jean Sanger, and Joseph Sanger. Part IV. Models of Morphogenesis and Development. Monoclonal Antibodies in the Analysis of Embryonic Development, Bedian. Mesoderm Induction in Xenopus: Oocyte Expression System and Animal Cap Assay, Yao, Kessler. Amphibian Organizer Activity, Niehrs. Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery, Packard, Cox,and Poole. Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration, Newgreen and Murphy. The Chimeric Human/Mouse Model of Angiogenesis, Petitclerc, von Schalscha, and Brooks. Analysis of Embryonic Vascular Morphogenesis, Sato and Bartunkova. Epithelial-Mesenchyme Interactions, Hall. Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning, Ros, Simandl, Clark, and Fallon. Palate Development--In Vitro Procedures, Pisano and Greene. Part V. In Vitro Models and Analysis Differentiation and Development. In Vitro Fertilization Heyner, Tucker. Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development, Peters, Chapman, and Soares. Bone Marrow-Derived Mesenchymal Progenitor Cells, Johnstone and Yoo. Identification, Characterization, and Differentiation of Human Prostate Cells, Mehta, Perez-Stable, Roos, and Nadji. Preparation of Chick Striated Muscle Cultures, DiLullo, George-Weinstein, and Gerhart. Study of Skeletal Myogenesis in Cultures of Unsegmented Somitic Mesoderm, Borycki and Emerson. Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Chondrocyte Maturation, DeLise, Stringa, Woodward, Mello, and Tuan. Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells, DeLise and Tuan. Murine Cells, Haas and Tuan. Skeletogenesis: In Vitro Analysis of Bone Cell Differentiation, Majolagbe and Robey. Studying Early Hematopoiesis Using Avian Blastoderm Cultures, Eisenberg. Isolation and Culture of Mouse Germ Cells, De Miguel and Donovan. Cadherin-Mediated Cell-Cell Interactions, Knudsen and Soler. Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Progeins, Underhill and Zhang. Microinjection of Fluorescently Labeled Alpha-Actinin into Living Cells, Jean Sanger, Danowski, and Joseph Sanger. Pax-3 and Vertebrate Development, Epstein. Genetic-Engineered Models of Skeletal Diseases I. Collagen Type X, Jacenko.

Developmental biology protocols

著者

書誌事項

大学図書館所蔵件 / 全26件

この図書・雑誌をさがす

注記

内容説明・目次

関連文献： 1件中 1-1を表示

詳細情報

書き出し