Flow cytometry and cell sorting
著者
書誌事項
Flow cytometry and cell sorting
（Springer lab manual）
Springer Verlag, 1999
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories.
A Springer Lab Manual Review of the First Edition:
"This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended."
CYTOBIOS
目次
- Preface - Leonard A. Herzenberg
- Part I Flow Cytometry: Operation of a Flow Cytometer - Christoph Goettlinger, Birgit Mechtold and Andreas Radbruch
- Part II Immunofluorescence: 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies - Werner Muller
- 3 Immunofluorescence: Basic Considerations - Andreas Radbruch
- 4 Multicolor Immunofluorescence Analysis - Chia-Huei Chen, Kerstin Willmann, Dieter Recktenwald
- 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells - Mario Assenmacher
- 6 Cytometry and Sorting of Live Cells According to Their Secreted Products - Rudolf A. MANZ
- 7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes - Alexander Scheffold
- 8 Scatchard Analysis by Flow Cytometry - Robert F. Murphy
- 9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry - Attila Trnok, Ursel Noehrenberg, Hans-Jurgen Vollmer, Stephan Schuhmacher
- Part III DNA and Proliferation: 10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis - Friedrich J. Otto
- 11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression - Werner Muller
- 12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique - Manfred Kubbies
- Part IV Cellular Activation and Biochemistry: 13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium- Manfred Kubbies
- 14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry - Attila Tarnok and Henning Ulrich
- 15 Biochemical Parameters of Cell Function - Gregor Rothe and Gunther Valet
- 16 The FACS-Gal Assay - Steffen Jung
- 17 Ligand Acidification by Nonadherent Cells - Robert F. Murphy
- Part V Cell Sorting: 18 Powerful Preselection - Charlotte Esser
- 19 Magnetic Selection of Transfected Cells - Gregor Siebenkotten and Ute Behrens-Jung
- 20 High Gradient Magnetic Cell Sorting - Stefan Miltenyi andJurgen Schmitz
- 21 Setup of a Flow Sorter - Christoph Goettlinger, Birgit Mechtold, Klaus Meyer and Andreas Radbruch
- 22 Sorting of Rare Cells - Walter Weichel, Sigrid Irlenbusch, Kimitaka Kato and Andreas Radbruch
- 23 Fluorescence-Activated Chromosome Sorting - Judith A. Fantes and Daryll K. Green
- 24 Analysis and Sorting of Plant Chromosomes by Flow Cytometry - Jacky Veuskens, Dominque Marie, Steph Hinnisdaels, Spencer C. Brown
- 25 Large Particle Sorting - David W. Galbraith and Sergio Lucretti
- Part VI Saftey: 26 Biological and Laser Safety - Klaus L. Meyer
- Appendices: Glossary
- Suppliers
- Cytometry on the Internet
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