書誌事項

Plant hormone protocols

edited by Gregory A. Tucker and Jeremy A. Roberts

(Methods in molecular biology / John M. Walker, series editor, v. 141)

Humana Press, c2000

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Established investigators from around the world describe in step-by-step detail their best techniques for the study of plant hormones and their regulatory activities. These state-of-the-art methods include contemporary approaches to identifying the biosynthetic pathways of plant hormones, monitoring their levels, characterizing the receptors with which they interact, and analyzing the signaling systems by which they exert their effects. Comprehensive and fully detailed for reproducible laboratory success, Plant Hormone Protocols offers plant biologists an indispensable compendium of today's most powerful methods and strategies to studying plant hormones, their regulation, and their activities.

目次

Extraction and Purification of an Enzyme Potentially Involved in ABA Biosynthesis, Gregory A. Tucker, Pete Bass, and Ian Taylor. Differential Display: Analysis of Gene Expression During Plant Development, Catherine A. Whitelaw, Benedetto Ruperti, and Jeremy A. Roberts. Abscisic Acid: ABA Immunoassay and Gas Chromatography/Mass Spectrometry Verification, M. K. Walker-Simmons, Patricia A. Rose, Lawrence R. Hogge, and Suzanne R. Abrams. Auxin Analysis, Els Prinsen, Stijn Van Laer, Sevgi A-den, and Henri Van Onckelen. Photoacoustic and Photothermal Detection of the Plant Hormone Ethylene, Laurentius A. C. J. Voesenek, Frans J. M. Harren, Hugo S. M. de Vries, Cor A. Sikkens, Sacco te Lintel Hekkert, and Cornelius W. P. M. Blom. Analysis of Gibberellins, Stephen J. Croker and Peter Hedden. Cytokinins: Extraction, Separation, and Analysis, Paula E. Jameson, Huaibi Zhang, and David H. Lewis. Binding Studies, Michael A. Venis. Mutagenesis, Ottoline Leyser. The Identification of Ethene Biosynthetic Genes by Gene Silencing: Antisense Transgenes, Agrobacterium-Mediated Transformation, and the Tomato ACC Oxidase cDNA, Grantley W. Lycett. Extraction, Separation, and Analysis of Plant Phosphoinositides and Complex Glycolipids, Bjorn K. Drobak, Nicholas J. Brewin, and Luis E. Hernandez. Reverse Genetics: Screening Plant Populations for Gene Knockouts, Sean T. May, Deborah Clements, and Malcolm J. Bennett. Index.

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詳細情報

  • NII書誌ID(NCID)
    BA46997639
  • ISBN
    • 0896035778
  • LCCN
    99058889
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    Totowa, N.J.
  • ページ数/冊数
    x, 199 p.
  • 大きさ
    24 cm
  • 親書誌ID
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