Spectrophotometry & Spectrofluorimetry : a practical approach
著者
書誌事項
Spectrophotometry & Spectrofluorimetry : a practical approach
（The practical approach series, 225）
Oxford University Press, c2000
- :hbk.
- :pbk.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume is intended to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day-to-day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications. These include spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phoshorescence studies, circular dichroism and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability.
目次
- Introduction to light absorption
- visible and ultraviolet spectra
- Fluorescence principles and measurement
- Time resolved fluorescence and phosphorescence spectroscopy
- An introduction to circular dichroism
- Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods
- Steady state kinetics
- Spectrophotometric assays
- Stopped flow spectroscopy
- Stopped flow fluorescence
- Stopped flow circular dichroism
- Spectrophotometry and fluorimetry of cellular compartments and intracellular processes
- Use of optical spectroscopic methods to study the thermodynamic stability of proteins
- The use of spectroscopic techniques in the study of protein stability.
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