Flow cytometry : a practical approach

著者

• Ormerod, Michael G.

書誌事項

Flow cytometry : a practical approach

edited by Michael G. Ormerod

（The practical approach series, 229）

Oxford University Press, c2000

3rd ed.

• :hbk.
• :pbk.

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

:pbk. ISBN 9780199638246

内容説明

Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

目次

• Introduction to the principles of flow cytometry
• Fluorescence and fluorescence technology
• Preparing of suspensions of single cells
• Flow sorting
• Immunofluorescence of surface markers
• Analysis of DNA - general methods
• Further clinical applications
• Quality control in the clinical laboratory
• Measurement of cytoplasmic and nuclear antigens
• Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method
• Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method
• Chromosome analysis and sorting by flow cytometry
• Intracellular ionised calcium, magnesium, membrane potential and pH
• Flow cytometry in the study of apoptosis
• Further applications to cell and molecular biology

巻冊次

:hbk. ISBN 9780199638253

内容説明

This text is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter of immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. The text aims to cover over 90 per cent of all applications of flow cytometry in mammalian biology.

目次

• Introduction to the principles of flow cytometry
• fluorescence and fluorescence technology
• preparing of suspensions of single cells
• flow sorting
• immunofluorescence of surface markers
• analysis of DNA - general methods
• further clinical applications
• quality control in the clinical laboratory
• measurement of cytoplasmic and nuclear antigens
• analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method
• analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method
• chromosome analysis and sorting by flow cytometry
• intracellular ionised calcium, magnesium, membrane potential and pH
• flow cytometry in the study of apoptosis
• further applications to cell and molecular biology.

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA4714569X
• ISBN:
  • 019963825X
  • 0199638241
• 出版国コード: uk
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Oxford
• ページ数/冊数: xx, 276 p.
• 大きさ: 25 cm
• 親書誌ID:
  • BA00227768