DNA-protein interactions : a practical approach
著者
書誌事項
DNA-protein interactions : a practical approach
(The practical approach series)
Oxford University Press, c2000
- : hbk
- : pbk
大学図書館所蔵 全21件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
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  フランス
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
DNA-Protein Interactions is a novel compilation of methods for studying the interactions of proteins with DNA. It is a rapidly advancing research area in which multidisciplinary approaches are especially valuable for solving problems and obtaining a detailed understanding of the molecular regulatory interactions involved. This book covers all the major tools that are required for the study of the large macromolecular enzymatic machines that manipulate DNA,
with particular emphasis on biophysical techniques applied to the analysis of transcription and its relation to chromatin structure. Knowledge of basic techniques is assumed, although advances in fundamental fields are covered.
目次
- Expression Systems
- Gel electrophoresis and bending in cisplatin-modified linear DNA
- Use of DNA microcircles in protein-DNA binding studies
- Use of topology to measure protein-induced DNA bend and unwinding angles
- Bandshift, gel retardation, or electrophoretic mobility shift assays
- Practical aspects of Fluorescence Resonance Energy Transfer (FRET) and its applications in nucleic acid biochemistry
- Determination of DNA-ligand interactions by fluorescence correlation spectroscopy
- DNA wrapping in E. Coli RNA polymerase open promotor complexes revealed by scanning force microscopy
- Microcalorimetry of protein-DNA interactions
- DNA-protein cross-linking by formaldehyde: a probe for RNAP-open promoter complex structure
- Solid-phase DNAse I footprinting
- Hydroxyl radical footprinting
- Radiolytic cleavage of DNA: Mapping of the Protein Interaction Sites
- UV - Laser photoreactivity of nucleoprotein complexes in vitro
- In vivo UV-laser footprinting
- Digitization and quantitative analysis of footprinting gels
- Mapping histone positions in chromatin by protein-directed DNA cross-linking and cleavage
- Kinetics of DNA-protein interactions
- Kinetics of DNA interactions studied by surface plasmon resonance spectroscopy
- Quantitative DNase I Kinetics Footprinting
- Real time fluorescence anisotropy for studying protein-DNA interactions
- Analysis of protein-DNA interactions in complex nucleoprotein assemblies
- Site-specific protein-DNA photocrosslinking
- Visualization of protein-DNA complexes by cryo em and on grids
- Characterization of T7 RNA polymerase protein-DNA interactions during the initiation and elongation phases
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