The ELISA guidebook
著者
書誌事項
The ELISA guidebook
(Methods in molecular biology / John M. Walker, series editor, v. 149)
Humana Press, c2001
- :hbk.
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
ISBN 9780896037281
内容説明
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
目次
Overview of ELISA in Relation to Other Disciplines
Systems in ELISA
Stages in ELISA
Titration of Reagents
Theoretical Considerations
Practical Exercises
Monoclonal Antibodies
Validation of Diagnostic Tests for Infectious Diseases
Charting Methods for Internal Quality Control
Immunochemical Techniques
Test Questions
- 巻冊次
-
:hbk. ISBN 9780896039506
内容説明
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
目次
Overview of ELISA in Relation to Other Disciplines. Systems in ELISA. Stages in ELISA. Titration of Reagents. Theoretical Considerations. Practical Exercises. Monoclonal Antibodies. Validation of Diagnostic Tests for Infectious Diseases. Charting Methods for Internal Quality Control. Immunochemical Techniques. Test Questions.
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