Experimental protocols for reactive oxygen and nitrogen species
著者
書誌事項
Experimental protocols for reactive oxygen and nitrogen species
Oxford University Press, 2000
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Experimental Protocols for Reactive Oxygen and Nitrogen Species is a translated, expanded, and fully updated of the Japanese book Experimental Protocols for Reactive Oxygen Research: assay methods, gene analysis, and pathophysiology models published in 1994. The aim of the book is to provide experimental protocols covering many aspects of free radical research: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The
protocols are all self-contained describing the equipment and reagents needed and then detailing the experimental procedure.
目次
- PART ONE: MEASUREMENT
- ROS and RNS measurements
- Measurements for proteinaceous plasma antioxidants, low Mw antioxidants, and REDOX related compounds
- Measurements for specific ROS and RNS enzymes and related proteins
- Lipids and lipid peroxidization
- Iron and reactive iron species
- Copper and reactive copper species
- PART TWO: GENE ANALYSES
- Research for gene expressions of ROS and RNS related genes
- DNA damage and repair
- PART THREE: EXPERIMENTS FOR PATHOPHYSIOLOGICAL MODELS
- Isolated and perfused hearts (Langendorf's model)
- Myocardial ischaemia-reperfusion models in vivo
- GI tracts in vivo
- Brain ischaemia
- Cell culture
- Athersclerosis
- Dicarbonyls and HB-EGF gene expression UV damage
- Ageing
- Ovulation
- Scavenging NO in experimental animals
- Using radioimmuno assays in brain slices
- Oxidative stress
- Carcinogenesis
- Iron overload
- Copper overload
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