Experimental protocols for reactive oxygen and nitrogen species

書誌事項

Experimental protocols for reactive oxygen and nitrogen species

edited by Naoyuki Taniguchi and John M.C. Gutteridge

Oxford University Press, 2000

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Experimental Protocols for Reactive Oxygen and Nitrogen Species is a translated, expanded, and fully updated of the Japanese book Experimental Protocols for Reactive Oxygen Research: assay methods, gene analysis, and pathophysiology models published in 1994. The aim of the book is to provide experimental protocols covering many aspects of free radical research: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained describing the equipment and reagents needed and then detailing the experimental procedure.

目次

  • PART ONE: MEASUREMENT
  • ROS and RNS measurements
  • Measurements for proteinaceous plasma antioxidants, low Mw antioxidants, and REDOX related compounds
  • Measurements for specific ROS and RNS enzymes and related proteins
  • Lipids and lipid peroxidization
  • Iron and reactive iron species
  • Copper and reactive copper species
  • PART TWO: GENE ANALYSES
  • Research for gene expressions of ROS and RNS related genes
  • DNA damage and repair
  • PART THREE: EXPERIMENTS FOR PATHOPHYSIOLOGICAL MODELS
  • Isolated and perfused hearts (Langendorf's model)
  • Myocardial ischaemia-reperfusion models in vivo
  • GI tracts in vivo
  • Brain ischaemia
  • Cell culture
  • Athersclerosis
  • Dicarbonyls and HB-EGF gene expression UV damage
  • Ageing
  • Ovulation
  • Scavenging NO in experimental animals
  • Using radioimmuno assays in brain slices
  • Oxidative stress
  • Carcinogenesis
  • Iron overload
  • Copper overload

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