Rotaviruses : methods and protocols
Author(s)
Bibliographic Information
Rotaviruses : methods and protocols
（Methods in molecular medicine / John M. Walker, series editor, 34）
Humana Press, c2000
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
James Gray and Ulrich Desselberger have assembled a comprehensive collection of established and cutting-edge methods for studying and illuminating the structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of infant morbidity and mortality. Presented by experts in the fields of animal and human rotavirus infections and rotavirus vaccine research, these readily reproducible methods detail molecular and other modern techniques, and include relevant background information and various notes to ensure reproducible and robust results. Authoritative and up-to-date, Rotaviruses: Methods and Protocols offers researchers today's benchmark compendium of experimental methods for the investigation of this medically significant virus.
Table of Contents
Rotaviruses: Basic Facts, Ulrich Desselberger. Electron Cryomicroscopy and Computer Image Processing Techniques: Use in Structure-Function Studies of Rotavirus, B. V. Venkataram Prasad and Mary K. Estes. Virus Replication, John T. Patton, Vladimir Chizhikov, Zenobia Taraporewala, and Dayue Chen. Rotavirus Entry into Tissue Culture Cells, Joanna M. Gilbert and Harry B. Greenberg. Mixed Infections with Rotaviruses: Protocols for Reassortment, Complementation, and Other Assays, Robert F. Ramig. Pathogenesis and Animal Models, Linda J. Saif and Lucy A. Ward. Immunologic Methods and Correlates of Protection, Kristine K. Macartney and Paul A. Offit. In Vivo Study of Immunity to Rotaviruses: Selected Methods in Mice, Manuel A. Franco and Harry B. Greenberg. Evaluation of Rotavirus Vaccines in Small Animal Models, Max Ciarlet and Margaret E. Conner. Methods of Rotavirus Detection, Sero- and Genotyping, Sequencing, and Phylogenetic Analysis, Miren Iturriza Gomara, Jon Green, and Jim Gray. Epidemiology of Group A Rotaviruses: Surveillance and Burden of Disease Studies, Mary Ramsay and David Brown. Future Rotavirus Research, Ulrich Desselberger and Mary K. Estes. Index.
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