Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual

著者

書誌事項

Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual

Dan Burke, Dean Dawson, Tim Stearns

Cold Spring Harbor Laboratory Press, 2000

2000 ed

大学図書館所蔵 件 / 全21件

この図書・雑誌をさがす

注記

Rev. ed. of: Methods in yeast genetics / Alison Adams ... [et al.]. 1997 ed. 1998

Includes bibliographical references

内容説明・目次

内容説明

In the 2000 edition of this text, most of the experiments have been revised and several new experiments and methods have been added. These include: the 2-hybrid system, PCR-based methods and isolation of chromatin.

目次

  • Experiments: looking at yeast cells
  • isolation and characterization of auxotrophic, temperature-sensitive and UV-sensitive mutants
  • meiotic mapping
  • mitotic recombination and random spore analysis
  • transformation of yeast
  • synthetic lethal mutants
  • gene replacement
  • isolation of ras2 suppressors
  • manipulating cell types
  • isolating mutants by insertional mutagenesis
  • two-hybrid protein interaction method. Techniques and protocols: high-efficiency transformation of yeast
  • "Lazy Bones" plasmid transformation of yeast colonies
  • yeast DNA isolations
  • yeast DNA miniprep (40 ml)
  • yeast DNA miniprep (5 ml)
  • a ten-minute DNA preparation from yeast
  • yeast genomic DNA - glass bead preparation
  • yeast protein extracts
  • yeast RNA isolation
  • hydroxylamine mutagenesis of plasmid DNA
  • assay of beta-galactosidase in yeast
  • plate assay for carboxypeptidase Y
  • random spore analysis
  • yeast vital stains
  • yeast immunofluorescence
  • actin staining in fixed cells
  • PCR protocol for PCR-mediated gene disruption
  • yeast colony PCR protocol
  • measuring yeast cell density by spectrophotometry
  • cell synchrony
  • chromatin immunoprecipitation
  • flow cytometry of yeast DNA
  • logarithmic growth
  • EMS mutagenesis
  • tetrad dissection
  • making a tetrad dissection needle
  • picking zygotes
  • determining plating efficiency. Appendices: media
  • stock preservation
  • yeast genetic and physical maps
  • templates for making streak plates
  • electrophoretic karyotypes of strains for southern blot mapping
  • strains
  • counting yeast cells with a standard hemocytometer chamber
  • tetrad scoring sheet
  • trademarks
  • suppliers.

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