Essential molecular biology : a practical approach

著者

• Brown, T. A.

書誌事項

Essential molecular biology : a practical approach

edited by T. A. Brown

（The practical approach series）

Oxford University Press, 2000-2001

2nd ed.

• v.1 : hbk
• v.1 : pbk
• v.2 : hbk
• v.2 : pbk

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

v.1 : pbk ISBN 9780199636426

内容説明

The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 1 concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. It also includes a general introduction to molecular biology in the laboratory and a survey of cloning vectors for Escherichia Coli.

目次

• Getting Started in Molecular Biology
• Microbiological Techniques for Molecular Biology: bacteria and phages
• Purification of DNA
• Purification of RNA
• Nucleic Acid Electrophoresis in Agarose Gels
• Recovery of DNA from Electrophoresis Gels
• Construction of Recombinant DNA Molecules
• Generation and Identification of Recombinant Clones
• Survey of Cloning Vectors for Escherichia coli
• A1: Important Escherichia coli Strains
• A2: Recipes and General Procedures
• A3: Restriction Endonucleases

巻冊次

v.1 : hbk ISBN 9780199636433

内容説明

The two "Essential Molecular Biology" books in the "Practical Approach Series" are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 1 concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. It also includes a general introduction to molecular biology in the laboratory and a survey of cloning vectors for Escherichia Coli.

目次

Getting Started in Molecular Biology. Microbiological Techniques for Molecular Biology: bacteria and phages. Purification of DNA. Purification of RNA. Nucleic Acid Electrophoresis in Agarose Gels. Recovery of DNA from Electrophoresis Gels. Construction of Recombinant DNA Molecules. Generation and Identification of Recombinant Clones. Survey of Cloning Vectors for Escherichia coli. A1: Important Escherichia coli Strains. A2: Recipes and General Procedures. A3: Restriction Endonucleases

巻冊次

v.2 : pbk ISBN 9780199636440

内容説明

The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.

目次

• 1. Strategies for research in molecular biology
• 2. Construction of genomic libraries in lambda and cosmic vectors
• 3. Construction of a cDNA library
• 4. Nucleic acid labelling and detection
• 5. Immobilization of nucleic acids and hybridization analysis
• 6. DNA sequencing
• 7. The polymerase chain reaction
• 8. Protein expression in Escherichia coli
• 9. Transcript mapping
• 1. HEALTH HAZARDS AND SAFETY PROCEDURES
• 2. Equipment for molecular biology research
• 3. Important Escherichia coli strains
• 4. Recipes and general procedures
• 5. Restriction endonucleases
• 6. DNA and RNA modification enzymes
• 7. List of suppliers' addresses

巻冊次

v.2 : hbk ISBN 9780199636457

内容説明

The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.

目次

• 1. Strategies for research in molecular biology
• 2. Construction of genomic libraries in lambda and cosmic vectors
• 3. Construction of a cDNA library
• 4. Nucleic acid labelling and detection
• 5. Immobilization of nucleic acids and hybridization analysis
• 6. DNA sequencing
• 7. The polymerase chain reaction
• 8. Protein expression in Escherichia coli
• 9. Transcript mapping
• APPENDICES
• 1. Health hazards and safety procedures
• 2. Equipment for molecular biology research
• 3. Important Escherichia coli strains
• 4. Recipes and general procedures
• 5. Restriction endonucleases
• 6. DNA and RNA modification enzymes
• 7. List of suppliers' addresses

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA4971553X
• ISBN:
  • 0199636435
  • 0199636427
  • 0199636451
  • 0199636443
• 出版国コード: uk
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Oxford
• ページ数/冊数: 2 v.
• 大きさ: 25 cm
• 親書誌ID:
  • BA00227768