Essential molecular biology : a practical approach
著者
書誌事項
Essential molecular biology : a practical approach
（The practical approach series）
Oxford University Press, 2000-2001
2nd ed.
- v.1 : hbk
- v.1 : pbk
- v.2 : hbk
- v.2 : pbk
大学図書館所蔵 全21件
注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
v.1 : pbk ISBN 9780199636426
内容説明
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make
modifications to suit personal requirements. Volume 1 concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. It also includes a general introduction to molecular biology in the laboratory and a survey of cloning
vectors for Escherichia Coli.
目次
- Getting Started in Molecular Biology
- Microbiological Techniques for Molecular Biology: bacteria and phages
- Purification of DNA
- Purification of RNA
- Nucleic Acid Electrophoresis in Agarose Gels
- Recovery of DNA from Electrophoresis Gels
- Construction of Recombinant DNA Molecules
- Generation and Identification of Recombinant Clones
- Survey of Cloning Vectors for Escherichia coli
- A1: Important Escherichia coli Strains
- A2: Recipes and General Procedures
- A3: Restriction Endonucleases
- 巻冊次
-
v.1 : hbk ISBN 9780199636433
内容説明
The two "Essential Molecular Biology" books in the "Practical Approach Series" are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements. Volume 1 concentrates on the procedures for DNA and RNA manipulation: purification, electrophoresis, and the construction and cloning of recombinant molecules. It also includes a general introduction to molecular biology in the laboratory and a survey of cloning vectors for Escherichia Coli.
目次
Getting Started in Molecular Biology. Microbiological Techniques for Molecular Biology: bacteria and phages. Purification of DNA. Purification of RNA. Nucleic Acid Electrophoresis in Agarose Gels. Recovery of DNA from Electrophoresis Gels. Construction of Recombinant DNA Molecules. Generation and Identification of Recombinant Clones. Survey of Cloning Vectors for Escherichia coli. A1: Important Escherichia coli Strains. A2: Recipes and General Procedures. A3: Restriction Endonucleases
- 巻冊次
-
v.2 : pbk ISBN 9780199636440
内容説明
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications
to suit personal requirements.
Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.
目次
- 1. Strategies for research in molecular biology
- 2. Construction of genomic libraries in lambda and cosmic vectors
- 3. Construction of a cDNA library
- 4. Nucleic acid labelling and detection
- 5. Immobilization of nucleic acids and hybridization analysis
- 6. DNA sequencing
- 7. The polymerase chain reaction
- 8. Protein expression in Escherichia coli
- 9. Transcript mapping
- 1. HEALTH HAZARDS AND SAFETY PROCEDURES
- 2. Equipment for molecular biology research
- 3. Important Escherichia coli strains
- 4. Recipes and general procedures
- 5. Restriction endonucleases
- 6. DNA and RNA modification enzymes
- 7. List of suppliers' addresses
- 巻冊次
-
v.2 : hbk ISBN 9780199636457
内容説明
The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications
to suit personal requirements.
Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.
目次
- 1. Strategies for research in molecular biology
- 2. Construction of genomic libraries in lambda and cosmic vectors
- 3. Construction of a cDNA library
- 4. Nucleic acid labelling and detection
- 5. Immobilization of nucleic acids and hybridization analysis
- 6. DNA sequencing
- 7. The polymerase chain reaction
- 8. Protein expression in Escherichia coli
- 9. Transcript mapping
- APPENDICES
- 1. Health hazards and safety procedures
- 2. Equipment for molecular biology research
- 3. Important Escherichia coli strains
- 4. Recipes and general procedures
- 5. Restriction endonucleases
- 6. DNA and RNA modification enzymes
- 7. List of suppliers' addresses
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