Calcium signalling : a practical approach
著者
書誌事項
Calcium signalling : a practical approach
（The practical approach series, 246）
Oxford University Press, c2001
2nd ed
- : hbk
- : pbk
- 統一タイトル
-
Cellular calcium
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注記
Rev. ed. of: Cellular calcium / edited by James G. McCormack and P.H. Cobbold. c1991
Includes bibliographical references and index (p. 225-230)
内容説明・目次
- 巻冊次
-
: pbk ISBN 9780199638475
内容説明
This new edition of Calcium Signalling focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometric techniques are all covered in depth. Also described in detail are methods for monitoring mitochondrial function, measuring calcium in the nuclear envelope and nucleoplasm, measuring calcium dependent gene expression, monitoring calcium signals in multicellular preparations, and measuring calcium extrusion. A major problem of fluorescent calcium measurements is the artifacts from zinc and transition metals and a chapter is devoted to their detection and minimization. Some of the pharmacological methods of studying calcium release are also covered. Many of the chapters are written by the scientists who developed the methods they are describing so the authors could not be more qualified. It is hoped that this volume will prove as invaluable to the studying cellular calcium as its predecessor.
目次
- Chameleons as cytosolic and intra-organellar calcium probes
- Photometry, video imaging, confocal and multi-photon microscopy approaches in calcium signalling studies
- Detecting and minimizing errors in calcium-probe measurements arising from transition metals and zinc
- Targeting of bioluminescent probes and calcium measurements in different subcellular compartments
- Monitoring mitochondrial function in single cells
- Using low-affinity fluorescence calcium indicators and chelators for monitoring and manipulating free (Ca2+) in endoplasmic reticulum
- Measuring calcium in the nuclear envelope and nucleoplasm
- From calcium signal to gene expression. Controlling cytoplasmic calcium and measuring calcium-dependent gene expression in intact cells
- Monitoring the generation and propagation of calcium signals in multicellular preparations
- Pharmacological studies of new calcium release mechanisms
- Measuring calcium extrusion
- Appendix one - Fluorescent calcium indicators and caged calcium probes
- 巻冊次
-
: hbk ISBN 9780199638482
内容説明
This new edition of Calcium Signalling focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometric techniques are all covered in depth. Also described in detail are methods for monitoring mitochondrial function, measuring calcium in the nuclear envelope and
nucleoplasm, measuring calcium dependent gene expression, monitoring calcium signals in multicellular preparations, and measuring calcium extrusion. A major problem of fluorescent calcium measurements is the artifacts from zinc and transition metals and a chapter is devoted to their detection and minimization. Some
of the pharmacological methods of studying calcium release are also covered. Many of the chapters are written by the scientists who developed the methods they are describing so the authors could not be more qualified. It is hoped that this volume will prove as invaluable to the studying cellular calcium as its predecessor.
目次
- Chameleons as cytosolic and intra-organellar calcium probes
- Photometry, video imaging, confocal and multi-photon microscopy approaches in calcium signalling studies
- Detecting and minimizing errors in calcium-probe measurements arising from transition metals and zinc
- Targeting of bioluminescent probes and calcium measurements in different subcellular compartments
- Monitoring mitochondrial function in single cells
- Using low-affinity fluorescence calcium indicators and chelators for monitoring and manipulating free (Ca2+) in endoplasmic reticulum.
- Measuring calcium in the nuclear envelope and nucleoplasm
- From calcium signal to gene expression. Controlling cytoplasmic calcium and measuring calcium-dependent gene expression in intact cells.
- Monitoring the generation and propagation of calcium signals in multicellular preparations.
- Pharmacological studies of new calcium release mechanisms
- Measuring calcium extrusion
- Appendix one - Fluorescent calcium indicators and caged calcium probes
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