Molecular histochemical techniques
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Bibliographic Information
Molecular histochemical techniques
（Springer lab manual）
Springer, c2000
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Includes bibliographical references and index
Description and Table of Contents
Description
Detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. It describes in situ nick translation and TUNEL in detail, along with radioactive and non-radioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. The methodology is set out in easy-to-follow language by leading scientists working in the field today. Both a rich source of the latest information and a practical lab manual.
Table of Contents
1 Introduction to the Detection of DNA Strand Breaks.- 2 In Situ Nick Translation.- 3 In Situ Nick End-Labeling: Light Microscopical.- 4 In Situ Nick End-Labeling: Electron Microscopical.- 5 Introduction to the Detection of Specific DNA and RNA Sequences.- 6 In Situ Hybridization for DNA: Fluorescent Probe.- 7 In Situ Hybridization for RNA: Radioactive DNA Probe.- 8 In Situ Hybridization for RNA: Radioactive RNA Probe - A Protocol for Practice With a Kit.- 9 In Situ Hybridization for RNA: Nonradioactive probe: ds cDNA Probe.- 10 In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe.- 11 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: T-T Dimer.- 12 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (I).- 13 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (II).- 14 In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe.- 15 Electron Microscopic in Situ Hybridization and its Combination With Immunohistochemistry.- 16 Whole Mount in Situ Hybridization for mRNA detection in Chick Embryos.- 17 Introduction to the Detection of Transcription Regulatory Proteins.- 18 Southwestern Histochemistry.
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