Immune and receptor assays in theory and practice
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Bibliographic Information
Immune and receptor assays in theory and practice
CRC Press, c2000
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Includes bibliographical references and index.
Description and Table of Contents
Description
In the past ten years, employment of immuno- and receptor- assays has grown dramatically. Now used in the pharmaceutical industry for automated screening programs, in the agro-food industry for on-line processing control and food adulteration detection, and in clinical laboratories, they are fully integrated analytical tools. However, the literature often covers only one type of assay or just one of the many systems available. Immune and Receptor Assays in Theory and Practice gathers and organizes the available information to help you establish the best assay for your application.
This composite presents the fundamentals of both techniques and introduces practical examples of equation use, antibody and receptor purification, antigen labeling, immunization, and establishment of antibodies for long-term storage. It contrasts the many different assay designs and addresses market trends as the context for developing immuno-assay goals. In addition, this volume summarizes the biochemical and physical properties involved in antibody- and receptor-ligand interactions and reagent manufacture.
This is the first, single-volume synthesis of both immuno- and receptor-assays. With theoretical background and practical examples, Immune and Receptor Assays in Theory and Practice allows you to base your experiment on proven techniques, components, and applications for the most reliable results.
Table of Contents
Introduction. Receptors at Work. Receptors and Antibodies. Receptor and Antibody Production. Molecular Basis of Ligand-Receptor Interactions. Immuno- and Receptor-Assay Design. Analytical Signal: Generation and Detection. The Sample. Assay Validation. Data Analysis, Processing and Presentation.
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