Cancer metastasis : in vitro and in vivo experimental approaches
著者
書誌事項
Cancer metastasis : in vitro and in vivo experimental approaches
(Laboratory techniques in biochemistry and molecular biology, v. 29)
Elsevier, 2000
- cloth
- pbk.
- series
大学図書館所蔵 全15件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
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  フランス
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  オランダ
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The book describes most of the methods that are currently used in metastasis research. Both in vivo and in vitro protocols are illustrated, so that the metastatic process can be either analysed as a whole, or single events addressed separately. Each method is described in the frame of the metastatic process, therefore its significance and its limitations in the context of metastasis are always taken into account. Whenever possible, several alternative procedures are reported per each experimental issue, so that the researcher can choose the one that better suits her/his needs and possibilities.During the past 30 years a big effort has been made to elucidate the molecular mechanisms of cancer metastatis, the leading cause of death for cancer patients. A considerable number of assays have been set up, that can be used to address specific questions concerning the single metastatic steps, or can be applied to develop and test drugs specifically interfering with selected events during the metastatic spread. This book contains an exhaustive description of most of the methods and their rationale, that are currently used in metastatic research, both to analyse metastasis in its entirety (in vivo models), or to dissect the single steps of the metastatic process (in vitro assays).
目次
Preface. 1. Introduction. 2. Homotypic and Heterotypic Cell Adhesion in Metastasis. 2.1 Release of malignant cells from the tumor mass: intercellular cohesion. 2.2 Malignant tumor cells in the blood stream: interactions with blood. 2.3 Adhesion to the target organ. 3. Motility, Deformability and Metastasis. 3.1 Motility and metastasis. 3.2 The role of active and passive deformability in invasion and resistance to shear stress forces in the blood stream. 4. ECM Degradation and Invasion. 4.1 Degradation. 4.2 Invasion. 5. The Role of Growth Interactions in Cancer Metastasis. 5.1 Methods to evaluate growth interactions in vitro. 5.2 Growth interactions in vivo. 6. Selection of Metastatic Variants. 6.1 Selection of organ-specific metastatic variants. 6.2 Selection of metastatic variants with enhanced or decreased metastatic abilities. 7. Genetic Tagging as a Mean to Study Tumor Progression or Metastasis-related Genes. 7.1 Clonal dominance in tumor progression. 7.2 Visualization of cancer metastasis. 7.3 Genes controlling the metastatic phenotype: use of gene tags to identify metastasis-related genes. 8. In Vivo Cancer Metastasis Assays. 8.1 Why study metastasis in vivo? 8.2 What defines an appropriate model of metastasis? 8.3 Cell lines. 8.4 Considerations regarding animals. 8.5 Site of injection. 8.6 Materials needed. 8.7 Spontaneous metastasis assay. 8.8 Experimental metastasis assay. 8.9 Enumeration of metastases. 8.10 Statistical considerations. 8.11 The influence of stress. 8.12 Concluding remarks. 9. Angiogenesis and Metastasis. 9.1 The corneal assay for angiogenesis. 9.2 The chick embryo chorioallantoic membrane assay. 9.3 Subcutaneous implant assay. References.
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