Interpreting protein mass spectra : a comprehensive resource
著者
書誌事項
Interpreting protein mass spectra : a comprehensive resource
, Oxford University, 2000
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注記
"Developed and distributed in partnership by The American Chemical Society and Oxford University Press"--T.p. verso
Includes bibliographical references and index
内容説明・目次
内容説明
Interpreting Protein Mass Spectra is a hands-on laboratory guide for a wide range of researchers investigating the structures of proteins and peptides. The focus is on the interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). The book will also provide useful background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass
spectrometry (SIMS). The book includes numerous practical examples, detailed discussions of experimental setups, and valuable hints for troubleshooting both methods and the handling of materials.
目次
1: Electrospray Ionization as a Vehicle for Protein Mass Spectra
2: Analyte Separation Methods: High-Pressure Liquid Chromatography and Capillary Electrophoresis
3: The Isotope Effect at High Mass
4: Calculation of the Analyte Mass
5: Analytical Figures of Merit for a High-Mass Isotope Envelope
6: Isotope Distribution of a Multiply Charged Mass Spectral Peak
7: Experimental Parameters Affecting Electrospray Ionization Mass Spectra
8: The Cation Effect on Electrospray Ionization Mass Spectra
9: Interpretation of Peptide and Protein Mass Spectra
10: Interpretation of Phosphoprotein Mass Spectra
11: Immunological Applications: Proteins and Phosphoproteins
12: Interpretation of Glycoprotein Mass Spectra
Appendix 1
Appendix 2
Appendix 3
Glossary
Index
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