Basic molecular biology : essential techniques

Bibliographic Information

Basic molecular biology : essential techniques

Philippa D. Darbre

(Essential techniques series)

Wiley, c1999

  • : comb

Available at  / 5 libraries

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Note

Includes bibliographical references and index

Description and Table of Contents

Description

This manual offers an introduction to molecular biology. Containing an overview of the basic facts, chapters concentrate on simple protocols of the core molecular biology techniques, giving a theoretical outline, advice on problems, and the time scales needed to perform all of the steps.

Table of Contents

  • Preparation of DNA
  • preparation of RNA
  • preparation of Plasmid DNA
  • DNA analysis by restriction enzyme analysis and southern blotting
  • DNA analysis by PCR
  • RNA analysis by northern blotting
  • RNA analysis by RNA protection
  • RNA analysis by RT-PCR and differential display
  • analysis of DNA-protein interactions by gel shift assay
  • gene transfection. Appendices: recipes for routine solutions
  • source of reagents and equipment. (part contents)

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