Basic molecular biology : essential techniques
著者
書誌事項
Basic molecular biology : essential techniques
(Essential techniques series)
Wiley, c1999
- : comb
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This manual offers an introduction to molecular biology. Containing an overview of the basic facts, chapters concentrate on simple protocols of the core molecular biology techniques, giving a theoretical outline, advice on problems, and the time scales needed to perform all of the steps.
目次
- Preparation of DNA
- preparation of RNA
- preparation of Plasmid DNA
- DNA analysis by restriction enzyme analysis and southern blotting
- DNA analysis by PCR
- RNA analysis by northern blotting
- RNA analysis by RNA protection
- RNA analysis by RT-PCR and differential display
- analysis of DNA-protein interactions by gel shift assay
- gene transfection. Appendices: recipes for routine solutions
- source of reagents and equipment. (part contents)
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