Syphilis serology : principles and practice

著者

書誌事項

Syphilis serology : principles and practice

Gerald D. Wasley and Helen H.Y. Wong

(Oxford medical publications)

Oxford University Press, 1988

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注記

Bibliography: p. [143]-154

内容説明・目次

内容説明

This work is designed for anyone interested in the performance of serological tests for syphilis, but in particular laboratory worker. The authors recognize that there are variations in serological practice between laboratories and present here various methods which can be used for serological tests. The authors also include information on treponemal diseases, the related serological responses and the scientific basis of the medical laboratory technology involved in order to provide an understanding of the nature of the tests and the disease.

目次

  • Part 1: syphilis serology - historical. Part 2 The treponemal diseases of man: syphilis (sexually transmitted)
  • congenital syphilis
  • yaws
  • pinta
  • endemic syphilis. Part 3: modern disease control. Part 4 Microbiology of the treponemes: growth and structure
  • the Reiter treponeme. Part 5 The immune response: humoral response
  • treponemal antigens
  • antibodies associated with treponemal infections
  • cell-mediated response. Part 6: specimen reception and management. Part 7 Non-treponemal serological tests for syphilis: VDRL slide test
  • RPR test
  • carbon-VDRL antigen (automatic and manual)
  • other non-treponemal tests and their interpretation. Part 8 Microhaemagglutination technique: "Treponema pallidum" Haemagglutination Assay (TPHA). Part 9 Immunofluorescence technique: the indirect immunofluorescent antibody test and direct immunofluorescence test for "T. pallidum". Part 10: fluorescence microscopy applicable to syphilis serology. Part 11 Additional tests: Reiter Protein Complement Fixation Test (RPCFT)
  • "Treponema pallidum" Immobilization test (TPI). Part 12 Examination of CSF for neurosyphilis: tests for inflammatory process in the CNS and for excess globulins in CSF and for antibodies in CSF. Part 13 Quality control applicable to syphilis serology: Internal Quality Control (IQC)
  • External Quality Assurance (EQA). Part 14: laboratory skills. Part 15 The future development of syphilis serology: DNA technology
  • solid-phaze Haemadsorption (SPHA)
  • Enzyme-linked Immunosorbent Assay (ELISA)
  • immune blotting techniques. Appendices: preparation of FTA-abs test antigen
  • preparation of Reiter antigen
  • preparation of sorbent
  • preparation of Reiter sonicate
  • evaluation of FITC conjugates.

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