Imaging of nucleic acids and quantitation in photonic microscopy

著者

• Ronot, Xavier
• Usson, Yves

書誌事項

Imaging of nucleic acids and quantitation in photonic microscopy

edited by Xavier Ronot, Yves Usson

（Methods in visualization）

CRC Press, c2001

注記

Includes index

内容説明・目次

内容説明

Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cytometry, along with their uses in imaging and quantification fluorescence in in situ hybridization. Written by researchers with extensive experience in the use of photonic microscopy in nucleic acid research, the chapters examine such topics as: fixation; nucleotide substitution; fluorochrome properties; chemically modified nucleotides and methods of incorporation; single cell gel electrophoresis (comet assay) to detect DNA lesions; fluorescent imaging for in situ study of nuclear distribution and colocalization of molecules (FISH mapping); clarification of the fluorescence microscope and light captors; visualization and processing of 3-D data sets; image acquisition and quantification, along with examples; and more. The chapters were each written by authorities on the particular subjects. In a two-column format, the theory and protocol are placed alongside comments and observations resulting from their experience. The comprehensible explanations of concepts together with the detailed procedures make Imaging of Nucleic Acids and Quantitation in Photonic Microscopy indispensable to your understanding and application of these valuable techniques.

目次

Fluorochromes and Fluorescent Probes, Didier Grunwald and Xavier Ronot Nucleic Probes, Amaury du Moulinet d'Hardemare and Anne-Sophie Gauchez Comet Assay, Sophie Sauvaigo and Marie-Jeanne Richard High Resolution FISH Mapping, Karine Monier and Sophie Rousseaux Fluorescence Imaging, Yves Usson 3D Visualization and Image Processing, Yves Usson Fluorescence Image Analysis, Yves Usson Examples of Quantitation, Xavier Ronot and Yves Usson

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA53976366
• ISBN:
  • 0849308178
• LCCN: 00140132
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Boca Raton, FL
• ページ数/冊数: xv, 164 p.
• 大きさ: 26 cm
• 分類:
  • LCC : QP620
  • DC21 : 572.8
• 件名:
  • LCSH : Nucleic acids -- Analysis
  • LCSH : Fluorescent probes
  • LCSH : Fluorescence microscopy
• 親書誌ID:
  • BA53976333