Two-hybrid systems : methods and protocols
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Bibliographic Information
Two-hybrid systems : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 177）
Humana Press, c2001
- : hardcover
- : comb
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Includes bibliographical references and index
Description and Table of Contents
Description
Many, if not all, essential biological processes require selective interactions between proteins. Complex signaling systems require sequential, ordered protein-protein interactions at essentially all levels of the signaling cascade. For example, peptide hormones interact with selective membrane receptor proteins, and autophosphorylation of the receptor then recruits other key regulatory proteins that initiate kinase cascades in which each phosphorylation event requires selective recognition of the protein substrate. The ultimate signaling effect, in many cases, is the regulation of RNA polymerase II-directed transcr- tion in the nucleus, a process that involves numerous, multiprotein complexes important for transcription initiation, elongation, termination, and reinitiation. Defining, characterizing, and understanding the relevance of these protein- protein interactions is an arduous task, but substantial inroads have been made over the past 20 years.
The development of more recent methodologies, such as mammalian expression systems, immunopurification schemes, expression cloning strategies, surface plasmon resonance (BiaCore), and nanosequencing technologies, has contributed a wealth of new insights into these complex multiprotein mechanisms and clearly accelerated the discovery process. Arguably, the yeast two-hybrid system has been one of the predominant and most powerful tools in this discovery process.
Table of Contents
Part I. General Methods
The Two-Hybrid System: A Personal View
Stanley Fields and Paul L. Bartel
Growth and Maintenance of Yeast
Lawrence W. Bergman
Media Formulations for Various Two-Hybrid Systems
Michael Saghbini, Denise Hoekstra, and Jim Gautsch
Yeast Two-Hybrid Vectors and Strains
Philip James
High-Efficiency Transformation of Plasmid DNA into Yeast
Robin A. Woods and R. Daniel Gietz
Qualitative and Quantitative Assessment of Interactions
Monica M. Montano
Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies,
Alyson Byrd and Rene St-Arnaud
Part II. False Positives
Two-Hybrid System and False Positives: Approaches to Detection and Elimination
Ilya G. Serebriiskii and Erica A. Golemis
Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns
Dennis M. Kraichely and Paul N. MacDonald
Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones
Louie Naumovski
Part III. Applications
Smad Interactors in Bone Morphogenetic Protein Signaling
Xiangli Yang and Xu Cao
Protein Interactions Important in Eukaryotic Translation Initiation
Katsura Asano and Alan G. Hinnebusch
Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins
Sergio A. Onate
Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-Apoptosis Protein E1B-19K
Thirugnana Subramanian and G. Chinnadurai
Part IV. Alternative Strategies
Mammalian Two-Hybrid Assays: Analyzing Protein-Protein Interactions in the Transforming Growth Factor-_ Signaling Pathway
Xin-Hua Feng and Rik Derynck
One-Hybrid Systems for Detecting Protein-DNA Interactions
Mary Kate Alexander, Brenda D. Bourns, and Virginia A.Zakian
The Split-Hybrid System: Uncoding Multiprotein Networks and Defining Mutations That Affect Protein Interactions
Phyllis S. Goldman, Anthony J. DeMaggio, Richard H. Goodman, and Merl F. Hoekstra
Three-Hybrid Screens: Inducible Third-Party Systems
Bjoern Sandrock, Franck Tirode, and Jean-Marc Egly
Three-Hybrid Screens for RNA-Binding Proteins: Proteins Binding 3' End of Histone mRNA
Zbigniew Dominski and William F. Marzluff
Membrane Recruitment Systems for Analysis of Protein-Protein Interactions
Ami Aronheim
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