Biological centrifugation
著者
書誌事項
Biological centrifugation
(The basics)
Bios, 2001
- : pbk
大学図書館所蔵 全2件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes index
内容説明・目次
内容説明
An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques.
目次
Ch 1. PRINCIPLES AND STRATEGIES OF CENTRIFUGATION. Sedimentation of particles under the influence of gravity. Rate of sedimentation. Increasing the magnitude of the gravitational field. Differential centrifugation. Centrifugation in density gradients. Sedimentation coefficients and the svedberg equation. Ch 2. CENTRIFUGATION HARDWARE. Centrifuges - an overview. Basic rotor design. Rotor capacity and performance. Uses of centrifuges and rotors. Centrifuge rotor parameters. Centrifuge tubes. Good tube and rotor practice. Sedimentation of particles in rotors. Rotors for preformed density gradients. Zonal rotors. The analytical ultracentrifuge. Ch 3. GRADIENT MEDIA. Choosing a suitable density-gradient medium. Physicochemical characteristics of gradient media. Preparing solutions of gradient media. Analysis of fractions. Ch 4. GRADIENTS TECHNIQUES. Types of gradients and their uses. Pre-formed discontinuous gradients. Pre-formed continuous gradients. Nonlinear gradients. Self-generating gradients. Harvesting gradients. Ch 5. PURIFICATION OF MAMMALIAN CELLS. Introduction. Selection of medium. Handling of gradient media. Centrifugation strategy. Peripheral blood mononuclear cells. Human monocytes. Dendritic cells. Isolation of islets of langerhans from porcine pancreas. Stellate cells from mammalian liver. Other low-density cells. Ch 6. FRACTIONATION OF SUBCELLULAR ORGANELLES. Introduction. Homogenization of tissue or cells. Physical characteristics of subcellular particles. Differential centrifugation of a rat liver homogenate. Purification of organelles in gradients. Ch 7. FRACTIONATION OF MEMBRANE VESICLES. The membrane compartments. Density and size of membrane vesicles. Separation strategies. Endoplasmic reticulum. Golgi membranes. Plasma membrane and membrane domains. Isolation of vesicular membranes by density perturbation. Analytical gradients (secretion). Analytical gradients (endocytosis). Ch 8. FRACTIONATION OF MACROMOLECULES AND MACROMOLECULAR COMPLEXES. Buoyant density banding of nucleic acids and proteins. Rate-zonal (sedimentation-velocity) banding of proteins and ribonucleoproteins. Viruses. Plasma lipoproteins.
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