FISH : a practical approach

著者

    • Beatty, Barbara
    • Mai,Sabine
    • Squire, Jeremy

書誌事項

FISH : a practical approach

edited by Barbara Beatty, Sabine Mai and Jeremy Squire

(The practical approach series, 260)

Oxford University Press, 2002

  • : pbk

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内容説明・目次

巻冊次

ISBN 9780199638833

内容説明

This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labelling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics. The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics techniques in current use. This book will be of particular value to scientists, technologists, and students in the areas of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular diagnostics and genetics.

目次

  • INTRODUCTION
  • LABELLING METHODS
  • 3. FISH Mapping
  • 4. Murine FISH
  • 5. Fiber FISH
  • 6. RNA FISH
  • 7. Three Dimensional FISH
  • 8. CGH
  • 9. Clinical Cytogenetics
  • 10. Multicolour FISH and SKY
  • 11. cDNA Microarrays and FISH
巻冊次

: pbk ISBN 9780199638840

内容説明

This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labelling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics. The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics techniques in current use. This book will be of particular value to scientists, technologists, and students in the areas of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular diagnostics and genetics.

目次

  • 1. Introduction
  • 2. Labelling Methods
  • 3. FISH Mapping
  • 4. Murine FISH
  • 5. Fiber FISH
  • 6. RNA FISH
  • 7. Three Dimensional FISH
  • 8. CGH
  • 9. Clinical Cytogenetics
  • 10. Multicolour FISH and SKY
  • 11. cDNA Microarrays and FISH

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詳細情報

  • NII書誌ID(NCID)
    BA59163462
  • ISBN
    • 0199638837
    • 0199638845
  • 出版国コード
    uk
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 原本言語コード
    eng
  • 出版地
    Oxford
  • ページ数/冊数
    xvii, 255 p.
  • 大きさ
    25 cm
  • 分類
  • 件名
  • 親書誌ID
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