Patch-clamp analysis : advanced techniques

Author(s)

• Walz, Wolfgang
• Boulton, Alan A.
• Baker, Glen B.

Bibliographic Information

Patch-clamp analysis : advanced techniques

edited by Wolfgang Walz, Alan A. Boulton, Glen B. Baker

（Neuromethods, 35）

Humana Press, c2002

Note

Includes bibliographical references and index

Description and Table of Contents

Description

"Patch-Clamp Analysis: Advanced Techniques" describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.

Table of Contents

Technology of Patch-Clamp Electrodes. Whole-Cell Patch-Clamp Recordings. Single-Channel Recording. Combined Fluorometric and Electrophysiological Recordings. Patch-Clamp Techniques Applied to Brain Slices. Perforated Patch-Clamp Technique. Fast-Drug Application. Pipet Perfusion Methods: Techniques and Applications. Patch-Cram Detection of Cyclic GMP in Intact Cells. Loose-Patch Voltage-Clamp Technique. Recording from Macropatches. Combining Patch-Clamp Techniques with RT-PCR. Index.

by "Nielsen BookData"

Details

• NCID: BA59444891
• ISBN:
  • 1588290131
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Totowa, N.J
• Pages/Volumes: xii, 345 p.
• Size: 24 cm
• Classification:
  • DC21 : 571.6347
• Subject Headings:
  • LCSH : Patch-clamp techniques (Electrophysiology)
• Parent Bibliography ID:
  • BA07359406