Proteins and proteomics : a laboratory manual
著者
書誌事項
Proteins and proteomics : a laboratory manual
Cold Spring Harbor Laboratory Press, c2003
- : cloth
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: cloth ISBN 9780879695538
内容説明
Completion of sequencing of the human genome, as well as those of many other organisms, has opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. This book provides an authoritative and wide-ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, the book provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual aims to offer a useful laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype.
It should also be valuable for cell biologists, developmental biologists and neuroscientists exploring the intricacies of the proteome and a useful resource for protein chemists and biochemists.
目次
- Introduction to proteomics
- one-dimensional polyacrylamide gel electrophoresis
- preparing cellular and subcellular extracts
- preparative two-dimensional gel electrophoresis with immobilized pH gradients
- reversed-phase high -performance liquid chromatography
- amino- and carboxy-terminal sequence analysis
- peptide mapping and sequence analysis of gel-resolved proteins
- the use of mass spectrometry in proteomics
- proteomic methods for phosphorylation site mapping
- characterization of protein complexes
- making sense of proteomics - using bioinformatics to discover a protein's structure, functions, and interactions.
- 巻冊次
-
: pbk ISBN 9780879695545
内容説明
Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide-ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications.
This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.
目次
Chapter 1. Introduction to Proteomics Chapter 2. One-dimensional Polyacrylamide Gel Electrophoresis Chapter 3. Preparing Cellular and Subcellular Extracts Chapter 4. Preparative Two-dimensional Gel Electrophoresis with Immobilized pH Gradients Chapter 5. Reversed-phase High-performance Liquid Chromatography Chapter 6. Amino- and Carboxy-terminal Sequence Analysis Chapter 7. Peptide Mapping and Sequence Analysis of Gel-resolved Proteins Chapter 8. The Use of Mass Spectrometry in Proteomics Chapter 9. Proteomic Methods for Phosphorylation Site Mapping Chapter 10. Characterization of Protein Complexes Chapter 11. Making Sense of Proteomics: Using Bioinformatics to Discover a Protein's Structure, Functions, and Interactions
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