Manipulating the mouse embryo : a laboratory manual

著者

• Nagy, Andras
• Gertsenstein, Marina
• Vintersten, Kristina
• Behringer, Richard

書誌事項

Manipulating the mouse embryo : a laboratory manual

Andras Nagy ... [et al.]

Cold Spring Harbor Laboratory Press, c2003

3rd ed

• : cloth
• : pbk

注記

Other authors: Marina Gertsenstein, Kristina Vintersten, Richard Behringer

Includes bibliographical references and index

内容説明・目次

巻冊次

: cloth ISBN 9780879695743

内容説明

This edition offers a compilation of contemporary protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embyro and gamete cryop-reservation and rederivation, and gene expression. There is extensive background information on the use of these techniques. The "gold standard" techniques from previous editions for applying recombinant DNA technology to studies of mammalian embryonic development are also updated and recase, as is the summary of mouse development at a molecular level. This book offers a useful source of technical and theoretical guidance for mouse developmental biologists and geneticists and newcomers to these fields.

目次

• Developmental genetics and embryology of the mouse - past, present and future
• summary of mouse development
• production of transgenic and chimeric mice - general issues
• recovery and in vitro culture of pre implantation-stage embryos
• surgical procedures
• production of transgenic mice
• isolation and culture of blastocyst-derived stem cells
• vector designs for embyronic stem cell-based transgenesis and genome alterations
• introduction of foreign DNA into embryonic stem cells
• production of chimeras
• detection and analysis of the mouse - genome alterations and specific sequences
• parthenogenesis, pronclear transfer, and mouse cloning
• assisted reproduction
• cryopreservation of mouse embryos and germplasm
• techniques for visualizing gene products, cells, tissues, and organ systems
• setting up a micromanipulation laboratory.

巻冊次

: pbk ISBN 9780879695910

内容説明

Regarded as the Bible of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cutting-edge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The gold standard techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields. Key features of the third edition: New authors are leading authorities in mouse development and genetics, as were authors of prior editions. All new illustration and new design: All illustrations have been redrawn; extensive use of new illustrations and photographs of techniques and results; a new, contemporary design presents techniques more clearly. New explanatory text: More background information for techniques is presented as well as more extensive troubleshooting information. New, functional organization and techniques.

目次

Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future Chapter 2. Summary of Mouse Development Chapter 3. Production of Transgenic and Chimeric Mice: General Issues Chapter 4. Recovery and In Vitro Culture of Pre-implantation-Stage Embryos Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos Chapter 6. Surgical Procedures Chapter 7. Production of Transgenic Mice Chapter 8. Isolation, Culture, and Manipulation of Blastocyst-derived Stem Cells Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells Chapter 11. Production of Chimeras Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection Chapter 15. Cryopreservation, Rederivation, and Transport of Mice Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems Chapter 17. Setting up and Equipping a Micromanipulation Lab Appendices

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BA60478879
• ISBN:
  • 9780879695743
  • 9780879695910
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Cold Spring Harbor, N.Y.
• ページ数/冊数: viii, 764 p.
• 大きさ: 28 cm
• 件名:
  • LCSH : Mice -- Embryos -- Laboratory manuals
  • LCSH : Mice -- Genetic engineering -- Laboratory manuals
  • LCSH : Transgenic mice -- Laboratory manuals
  • LCSH : Mice as laboratory animals