Neural stem cells : methods and protocols
著者
書誌事項
Neural stem cells : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 198）
Humana Press, c2002
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
In "Neural Stem Cells: Methods and Protocols", internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.
目次
Part I. Isolation and Culture of NSCs Neural Differentiation of Embryonic Stem Cells K. Sue O'Shea Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens Eric D. Laywell, Valery G. Kukekov, Oleg Suslov, Tong Zheng, and Dennis A. Steindler Isolation of Stem and Precursor Cells from Fetal Tissue Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb Susan C. Barnett and A. Jane Roskams Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium Edmund Au and A. Jane Roskams Production of Immortalized Human Neural Crest Stem Cells Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, Myung A. Lee, and Jung H. Bang Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization Lamya S. Shihabuddin Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood Shijie Song and J. Sanchez-Ramos Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer Part II. Characterization of NSCs In Vitro A. Cellular Techniques Analysis of Cell Generation in the Telencephalic Neuroepithelium Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures Angelo L. Vescovi, Rossella Galli, and Angela Gritti Assessing the Involvement of Telomerase in Stem Cell Biology Mark P. Mattson, Peisu Zhang, and Weiming Fu Detection of Telomerase Activity in Neural Cells Karen R. Prowse In Vitro Assays for Neural Stem Cell Differentiation Marcel M. Daadi Electron Microscopy and Lac-Z Labeling Bela Kosaras and Evan Snyder B. Electrophysiological Techniques Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells David S. K. Magnuson and Dante J. Morassutti C. Molecular Techniques Fluorescence In Situ Hybridization Barbara A. Tate and Rachel L. Ostroff RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells Theresa E. Gratsch Differential Display: Isolation of Novel Genes Theresa E. Gratsch Cell Labeling and Gene Misexpression by Electroporation Terence J. Van Raay and Michael R. Stark Gene Therapy Using Neural Stem Cells Luciano Conti and Elena Cattaneo Modeling Brain Pathologies Using Neural Stem Cells Simonetta Sipione and Elena Cattaneo Part III. Utilization/Characterization of NSCs In Vivo A. Endogenous Pools of Stem/Progenitor Cells Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model Marcel M. Daadi Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods Yonehiro Kanemura, Shin-ichi Sakakibara, and Hideyuki Okano Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo Sanjay S. P. Magavi and Jeffrey D. Macklis Immunocytochemical Analysis of Neuronal Differentiation Sanjay S. P. Magavi and Jeffrey D. Macklis Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold Lisa A. Catapano, Sanjay S. P. Magavi, and Jeffrey D. Macklis B. Transplantation Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore Optimizing Stem Cell Grafting into the CNS Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields, Dante J. Morassutti, and David S. K. Magnuson Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos Lorenzo Magrassi Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells Tanja Zigova and Mary B. Newman Routes of Stem Cell Administration in the Adult Rodent Alison E. Willing, Svitlana Garbuzova-Davis Paul R. Sanberg, and Samuel Saporta
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