Interleukin protocols
Author(s)
Bibliographic Information
Interleukin protocols
(Methods in molecular medicine / John M. Walker, series editor, 60)
Humana Press, c2001
- alk. paper
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines. Collectively these proteins are the key orchestrators of host defense and the response to tissue injury. There are currently 23 different interleukins (numbered from IL-1 to IL-23), although the full extent of the interleukin family will only become clear upon analysis of the human genome sequence. Most important, interleukins are central to the pathogenesis of a wide range of diseases that involve an immune com- nent, including such conditions as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, psoriasis, and asthma. Interleukins have also been imp- cated in other conditions, including cancer, migraine, myocardial infarction, and depression. In essence, when cells are activated by interleukins, a program of gene expression is initiated in the target cell that alters the cell's phenotype, leading to enhanced immune reactivity, inflammation, and/or proliferation. Interleukins are therefore at the core of the cellular basis for many diseases. They are the subject of intense investigation by biomedical researchers and the targeting or use of interleukins in the clinic is proceeding apace. Approaches such as t- geting IL-4 in asthma or IL-1 in joint disease are being pursued, and it is likely that in the next 5-10 years a number of new therapies based on either inhib- ing or administering interleukins will be available.
Table of Contents
Part I: General Methods for Assaying Interleukin Protein and mRNA
ELISAs and Interleukin Research
Catherine Greene
ELISPOT Technique for Assaying Interleukins
Almuth Christine Hauer and Mona Bajaj-Elliott
Measurement of Interleukins by ELISPOT Assay with Particular Application for Dual Color Analysis (Stardust Assay)
Yoshihiro Okamato and Mikio Nishida
Assaying Interleukins Intracellularly by FACS
Liam O'Mahony and Maurice O'Donoghue
Basic RT-PCR for Measurement of Cytokine Expression
Mona Bajaj-Elliot and Almuth Christine Hauer
Quantitative PCR for Measurement of Cytokine Expression
Ellen M. Nilsen, Finn-Eirik Johnasen, and Per Brandtzaeg
Single Cell RT-PCR Method for the Detection of Cytokine mRNA
Moshe Ligumsky, Hovav Nechushtan, and Ehud Razin.
uantitation of Cytokine mRNA by Flash-Type Bioluminescence
Jeffrey K. Actor
Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method
Ginette Tardif, John A. Di Battista, Jean-Pierre Pelletier, and Johanne Martel-Pelletier
Quantification of Mouse IL-6 and TNF-_ mRNA Using Xplore Assays
Scott W. Van Arsdell, Kevin P. Murphy, Csaba Pazmany, Diane Erickson and Mark D. Moody
Measurement of Cytokine and Chemokine mRNA Using Nonisotopic MultiProbe RNase Protection Assay
Zheng-Ming Wang and Roman Dziarski
Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography
Terry M. Phillips and Benjamin F. Dickens
Detection of Cytokines by Immunohistochemistry
Mahmoud Huleihel
Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement
Nicolai Petrovsky
Part II: Illustration of Methodologies Using Individual Interleukins
Assaying Interleukin-8
Paul N. Moynagh
Measurement of Interleukin-2 by Bioassay
Lyanne Weston
Assaying Interleukin-15
Amos Douvdevani, Michal Weiler, and Eli Lewis
Part III: Assaying Interleukins in Particular Pathologies
Assaying Interleukin-6 in Breast Cancer
Judith Harmey, Amanda Haverty, Deirdre Foley and David Bouchier-Hayes
Cytokines, Stress, and Depressive Illness
Hymie Anisman, Shawn Hayley, Arun V. Ravindran, Zul Merali, and Jenna Griffiths
Measurement of Interleukins in Cutaneous Disorders
Khusru Asadullah, Antje Haeussler-Quade, Wolf Dietrich Doecke, Wolfram Sterry
Interleukins in Graves' Disease
Jan Komorowski
Evaluation of Cytokines in Migraine Patients by ELISA
Irene Munno, Mariarosaria Marinaro, Giuseppe Lacedra, Antonia Bassi, and Vincenzo Centonze
Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction
Radek Pudil
Part IV: Assaying Interleukins in Particular Biological Fluids
Measurement of Cytokines in Peritoneal Fluids
Reijo Punnonen and Juha Punnonen
Measurement of Cytokines in Seminal Plasma
Marek Glezerman, Eitan Lunenfeld, and Mahmoud Huleihel
Measurement of Cytokines in Induced Sputum: Application to the Investigation of Asthma and COPD
Peter G. Gibson and Jodie L. Simpson
Cytokines in Blister Fluid
Franco Ameglio and Luciano D'Auria
Measurement of Cytokines in Synovial Fluid
Fabrizio De Benedetti
Measurement of Cytokines in Cerebrospinal Fluid
Toshiyuki Yokoyama
Detection of Interleukins in Tears
Archana Thakur and Mark D. P. Willcox
Part V: Newer Techniques: Interleukin Signal Transduction, Recombinant Interleukin Production, Interleukin Gene Polymorphisms, and the use of cDNA Arrays
Measurement of Phosphoinositide 3-Kinase Activation by Interleukins
Stephen G. Ward
Analysis of Interleukin-2 S
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